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Chromosomal genes of the facultative methylotroph, Pseudomonas AM1, have been mobilized using the broad host-range plasmid, R68.45. Genes coding for C1 metabolism were transferred at a frequency of 10−5 to 10−4 per donor cell. The genes coding for resistance to cycloserine, phosphonomycin and streptomycin are linked to the methanol dehydrogenase gene, while the marker for thiamin auxotrophy is not.
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