1887

Abstract

Esterase isoenzyme patterns determined by slab gel electrophoresis were compared for nearly 60 isolates, particularly from the complex. Consistent differences were found between and , with the latter being characterized by having between one and five very fast moving esterase bands that were generally absent from the former. A range of asexual (unidentifiable) isolates, taken from the vicinity offish hatcheries or from fish lesions, were also examined and their esterase isoenzymes shown to be similar to those of , although usually showing fewer bands. The use of esterase isoenzymes for screening potential fish pathogenic isolates of is briefly compared with results obtained using oogonium morphology or cyst ornamentation, and the relative merits of each method are discussed. It is proposed that, on the basis of their distinctive cyst coat ornamentation (bundles of long ‘boathooks’) and esterase isoenzymes, fish lesion isolates can be distinguished from saprophytic isolates, and that analysis of isoenzymes should provide a useful method for the screening of potential pathogenic isolates.

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1983-08-01
2024-04-25
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References

  1. Beakes G.W. 1983; A comparative account of cyst coat ontogeny in saprophytic and fish lesion (pathogenic) isolates of the Saprolegnia diclina -parasiticacomplex. Canadian Journal of Botany 61:603–625
    [Google Scholar]
  2. Clare B.G., Zentmyer G.A. 1966; Starch gel electrophoresis of proteins from species of Phy-tophthora. . Phytopathology 56:1334–1335
    [Google Scholar]
  3. Coker W.C. 1923 The Saprolegniaceae with Notes on Other Water Molds. Chapel Hill: University of Carolina Press;
    [Google Scholar]
  4. Dick M.W. 1969; Morphology and taxonomy of the oomycetes, with special reference to Saprolegniaceae, Leptomitaceae and Phythiaceae. 1. Sexual reproduction. New Phytologist 68:751–775
    [Google Scholar]
  5. Dick M.W. 1973; Saprolegniales. In The Fungi, an Advanced Treatise. IV. A Taxonomic Review with Keys: Basidiomycetes and Lower Fungi p. 113–145 Ainsworth G.C., Sparrow F.K., Sussman A.S. Edited by New York & London: Academic Press.;
    [Google Scholar]
  6. Erselius L.J., Shaw D.S. 1982; Protein and enzyme differences between Phytophthora palmivoraand P. megakarya: evidence for self-fertilization in pairings of the two species. Transactions of the British Mycological Society 78:227–238
    [Google Scholar]
  7. Gill H.S., Powell D. 1968; The use of polyacrylamide gel disc electrophoresis in delimiting three species of Phytophthora. . Phytopathologische Zeitschrift 63:23–29
    [Google Scholar]
  8. Gill H.S., Zentmyer G.A. 1978; Identification of Phytophthora species by disc electrophoresis. Phytopathology 68:163–167
    [Google Scholar]
  9. Green B.R., Dick M.W. 1972; DNA base composition and taxonomy of the oomycetes. Canadian Journal of Microbiology 18:963–968
    [Google Scholar]
  10. Hall R. 1969; Molecular approaches to taxonomy of fungi. Botanical Review 35:285–304
    [Google Scholar]
  11. Hall R., Zentmyer G.A., Erwin D.C. 1969; Approach to taxonomy of Phytophthora through acrylamide gel-electrophoresis of proteins. Phytopathology 59:770–774
    [Google Scholar]
  12. Heath I.B., Greenwood A.D. 1970; Wall formation in the Saprolegniales II. Formation of cysts by the zoospores of Saprolegnia and Dictyuchus. . Archiv fur Mikrobiologie 75:67–79
    [Google Scholar]
  13. Johnson G.B. 1973; Enzyme polymorphism and biosystematics: the hypothesis of selective neutrality. Annual Review of Ecology and Systematics 4:93–116
    [Google Scholar]
  14. Johnson G.B. 1975; Use of internal standards in electrophoretic surveys of enzyme polymorphism. Biochemical Genetics 13:833–847
    [Google Scholar]
  15. Kaosiri T., Zentmyer G.A. 1980; Protein, esterase, and peroxidase patterns in the Phytophthora palmivora complex from cacao. Mycologia 78:988–1000
    [Google Scholar]
  16. Meyer J.A., Renard J.L. 1969; Protein and esterase patterns of two formae speciales of Fusarium oxysporum. . Phytopathology 59:1409–1411
    [Google Scholar]
  17. Nealson K.H., Garber E.D. 1967; An electrophoretic survey of esterases, phosphatases and leucine aminopeptidases in mycelial extracts of Aspergillus. . Mycologia 59:330–336
    [Google Scholar]
  18. Neish G.A. 1977; Observations on Saprolegnias of adult sockeye salmon, Oncorhynchus nerka (Wal- baum). Journal of Fish Biology 10:513–522
    [Google Scholar]
  19. Neish G.A., Green B.R. 1976; Nuclear and satellite DNA base composition and the taxonomy of Saprolegnia (Oomycetes). Journal of General Microbiology 96:215–219
    [Google Scholar]
  20. Peberdy J.F., Turner M. 1968; The esterases of Mortierella ramamiana in relation to taxonomy. Journal of General Microbiology 31:303–312
    [Google Scholar]
  21. Pickering A.D., Willoughby L.G. 1977; Epidermal lesions and fungal infections on the perch, Perea fluviatilis L. in Windermere. Journal of Fish Biology 11:349–354
    [Google Scholar]
  22. Pickering A.D., Willoughby L.G., Mcgrory C.B. 1979; Fine structure of secondary zoospore cyst cases in Saprolegnia isolates from infected fish. Transactions of the British Mycological Society 72:427–436
    [Google Scholar]
  23. Seymour R. 1970; The genus Saprolegnia. . Nova Hedwigia 19:1–24
    [Google Scholar]
  24. Shannon M.C., Ballal S.K., Harris J.W. 1973; Starch gel electrophoresis of enzymes from nine species of Polyporus. . American Journal of Botany 60:96–100
    [Google Scholar]
  25. Stout D.L., Shaw C.R. 1974; Genetic distance among certain species of Mucor. Mycologia 66:969–977
    [Google Scholar]
  26. Vazira-Teherani B., Dick M.W. 1980a; Amino acid composition of oomycete cell walls. Transactions of the British Mycological Society 74:225–230
    [Google Scholar]
  27. Vazira-Teherani B., Dick M.W. 1980b; Taxonomic significance of variations between ratios of cell wall amino acids in fungi, with special reference to oomycete fungi. Transactions of the British Mycological Society 74:231–238
    [Google Scholar]
  28. Whitney P.J., Vaughan J.G., Heale JB. 1967; A disc electrophoretic study of proteins of Verticil- Hum albo-atrum, Verticillium dahliae, and Fusarium oxysporum with reference to their taxonomy. Journal of Experimental Botany 19:415–426
    [Google Scholar]
  29. Willoughby L.G. 1978; Saprolegnias of salmonid fish in Windermere: a critical analysis. Journal of Fish Diseases 1:51–67
    [Google Scholar]
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