Identification of Entomocidal Toxins of thuringiensis by High Performance Liquid Chromatography Free

Abstract

Entomocidal proteins produced by certain subspecies of were isolated from purified crystals (parasporal bodies) by column chromatography using Sephacryl S-300. The crystals of most subspecies contained only one entomocidal protein (P-1), but some strains of which had been classified as subsp. , subsp. , subsp. and subsp. appeared to produce an additional protein (P-2) as a minor component of the crystal. The protein (P-2) was serologically similar to the mosquito factor previously discovered in the HD-1 strain of subsp. These entomocidal proteins (P-1 and P-2) were isolated and digested by trypsin. The peptides resulting from the trypsin digestion were mapped by high performance liquid chromatography (HPLC). HPLC patterns of the toxins, in particular P-1, were reliably reproducible and revealed differences in P-1 toxins between strains even in the same serotype. Analysis of P-2 by HPLC indicated that it is different from P-1 in protein structure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-8-2595
1983-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/8/mic-129-8-2595.html?itemId=/content/journal/micro/10.1099/00221287-129-8-2595&mimeType=html&fmt=ahah

References

  1. Chestukhina G.G., Kostina L.I., Mikhailova A.L., Tyurin S.A., Klepikova F.S., Stepanov V.M. 1982; The main features of Bacillus thuringiensis δ-endotoxin molecular structure. Archives of Microbiology 132:159–162
    [Google Scholar]
  2. De Barjac H., Bonnefoi A. 1973; Classification of Bacillus thuringiensis. . Entomophaga 18:5–17
    [Google Scholar]
  3. Dulmage H.T. 1981; Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control. In Microbial Control of Pests and Plant Diseases 1970-1980 pp. 191–220 Edited by Burges H.D. London: Academic Press;
    [Google Scholar]
  4. Fullmer C.S., Wasserman R.H. 1979; Analytical peptide mapping by high performance liquid chromatography. Application to intestinal calciumbinding proteins. Journal of Biological Chemistry 254:7208–7212
    [Google Scholar]
  5. Goldberg L.J., Margalit J. 1977; A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univitattus, Aedes aegypti and Culex pipiens. . Mosquito News 37:355–358
    [Google Scholar]
  6. Gonzalez J.M. Jr Carlton B.C. 1982; Plasmid transfer in Bacillus thuringiensis. . In Genetic Exchange. A Celebration and A New Generation pp. 85–95 Edited by Streips U. N., Goodgal S.H., Guild W.R., Wilson G.A. New York & Basel: Marcel Dekker;
    [Google Scholar]
  7. Hall I.M., Arakawa K.Y., Dulmage H.T., Correa J.A. 1977; The pathogenicity of strains of Bacillus thuringiensis to larvae of Aedes and to Culexmosquitoes. Mosquito News 37:246–251
    [Google Scholar]
  8. Huber H.E., Luthy P., Ebersold H.-R., Cordier J.-L. 1981; The subunits of the para- sporal crystal of Bacillus thuringiensis: size, linkage and toxicity. Archives of Microbiology 129:14–18
    [Google Scholar]
  9. Lilley M., Ruffell R.N., Somerville H.J. 1980; Purification of the insecticidal toxin in crystals of Bacillus thuringiensis. . Journal of General Microbiology 118:1–11
    [Google Scholar]
  10. Yamamoto T., Mclaughlin R.E. 1981; Isolation of a protein from the parasporal crystal of Bacillus thuringiensis var. kurstaki toxic to the mosquito larva; Aedes taeniorhynchus. . Biochemical and Biophysical Research Communications 103:414–421
    [Google Scholar]
  11. Yamamoto T., Garcia J.A., Dulmage H.T. 1983; Immunological properties of entomocidal protein of Bacillus thuringiensis and its insecticidal activity. Journal of Invertebrate Pathology 41:122–130
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-8-2595
Loading
/content/journal/micro/10.1099/00221287-129-8-2595
Loading

Data & Media loading...

Most cited Most Cited RSS feed