1887

Abstract

The general control of amino acid biosynthesis was shown to play an important role in the coordination between cell growth and cell division under amino acid limitations. Mutant strains defective in this regulatory system, as studied here mainly with mutant strain RH375 (), showed excessive and aberrant cell growth under mild limitation, and rapid loss of cell viability under severe amino acid limitation. Furthermore, wild-type () cells were able to derepress, or at least maintain, levels of enzymes subject to the general control under amino acid limitations. The mutant cells showed significantly decreased enzyme levels under these conditions. The loss of viability of mutant cells was not due to inability to accumulate at ‘Start’ under amino acid limitation. In conclusion, we postulate that the aberrant behaviour of -mutant cells is due to an inability to maintain adequate levels of amino acid biosynthetic enzymes throughout the mitotic cell cycle.

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1983-08-01
2021-07-28
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References

  1. Amerine M. A., Ough C. S. 1980 Methods for Analysis of Musts and Wines p. 154 New York & London: Wiley;
    [Google Scholar]
  2. Bestic P. B., Arnold W. N. 1976; Linear transformation of standard curves for yeast turbidity. Applied and Environmental Microbiology 32:640–641
    [Google Scholar]
  3. Brenner M., Ames B. N. 1971; The histidine regulation: tryptophan mediated control of histidine operon and its regulation. In Metabolic Pathways, and arginine biosynthetic enzymes in Neurospora vol. 5, Metabolic Regulation, 3rd edn. pp. 349–387 Vogel H. J. Edited by London: Academic Press;
    [Google Scholar]
  4. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. 1973; Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor. Experimental Cell Research 76:99–110
    [Google Scholar]
  5. Carsiotis M., Jones R. F. 1974; Cross pathways regulation: tryptophan mediated control of histidine and arginine biosynthetic enzymes in Neurospora crassa. Journal of Bacteriology 119:889–892
    [Google Scholar]
  6. Carter B. L. A., Jagadish M. N. 1978; The relation between cell size and cell division in the yeast Saccharomyces cerevisiae. Experimental Cell Research 112:15–23
    [Google Scholar]
  7. Delforge J., Messenguy F., Wiame J. M. 1975; The regulation of arginine biosynthesis in Saccharomyces cerevisiae. The specificity of arg R-mutations and the general control of amino acid biosynthesis. European Journal of Biochemistry 57:231–239
    [Google Scholar]
  8. Fowell R. R. 1966; Hybridization of yeast. Process Biochemistry 1:25–28
    [Google Scholar]
  9. Hartwell L. H., Unger M. V. 1977; Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. Journal of Cell Biology 75:422–235
    [Google Scholar]
  10. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  11. Hilger F., Culot M., Minet M., Pierard A., Grenson M., Wiame J. M. 1973; Studies on the kinetics of the enzyme sequence mediating arginine synthesis in Saccharomyces cerevisiae. Journal of General Microbiology 75:33–41
    [Google Scholar]
  12. Holley R. W. 1975; Control of growth of mammalian cells in cell culture. Nature, London 258:487–490
    [Google Scholar]
  13. Hsu Y.-P., Kohlhaw G. B., Niederberger P. 1982; Evidence that a-isopropylmalate synthase of Saccharomyces cerevisiae is under the ‘general’ control of amino acid biosynthesis. Journal of Bacteriology 150:969–972
    [Google Scholar]
  14. Johnston G. C., Pringle J. R., Hartwell L. H. 1977; Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Experimental Cell Research 105:79–98
    [Google Scholar]
  15. Johnston J. R., Mortimer R. K. 1959; Use of snail digestive juice in isolation of yeast spore tetrads. Journal of Bacteriology 78:292
    [Google Scholar]
  16. Mclaughlin C. S., Magee P. T., Hartwell L. H. 1969; Role of isoleucyl-transfer ribonucleic acid synthetase in ribonucleic acid synthesis and enzyme repression in yeast. Journal of Bacteriology 100:579–584
    [Google Scholar]
  17. Miozzari G., Niederberger P., Hutter R. 1978a; Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway. Journal of Bacteriology 134:48–59
    [Google Scholar]
  18. Miozzari G., Niederberger P., Hütter R. 1978b; Permeabilization of microorganisms by Triton X-100. Analytical Biochemistry 90:220–223
    [Google Scholar]
  19. Niederberger P., Miozzari G., Hütter R. 1981; Biological role of the general control of amino acid biosynthesis in Saccharomyces cerevisiae. Molecular and Cellular Biology 1:584–593
    [Google Scholar]
  20. Pardee A. B. 1974; A restriction point for control of normal animal cell proliferation. Proceedings of the National Academy of Sciences of the United States of America 71:1286–1290
    [Google Scholar]
  21. Piotrowska M. 1980; Cross-pathway regulation of ornithine carbamoyltransferase synthesis in Aspergillus nidulans. Journal of General Microbiology 116:335–339
    [Google Scholar]
  22. Pringle J., Hartwell L. H. 1981; The Saccharomyces cerevisiae cell cycle. In The Molecular Biology of the Yeast Saccharomyces pp. 97–142 Edited by Strathern J. N., Jones E. W., Broach J. R. Cold Spring Harbor,New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Schürch A., Miozzari J., Hütter R. 1974; Regulation of tryptophan biosynthesis in Saccharomyces cerevisiae’. mode of action of 5-methyl- tryptophan and 5-methyl-tryptophan-sensitive mutants. Journal of Bacteriology 117:1131–1140
    [Google Scholar]
  24. Shilo B., Simchen G., Pardee A. B. 1978; Regulation of cell-cycle initiation in yeast by nutrients and protein synthesis. Journal of Cell Physiology 97:177–187
    [Google Scholar]
  25. Stewart P. R. 1975; Analytical methods in yeasts. Methods in Cell Biology 12:111–147
    [Google Scholar]
  26. Tyson C. BLORD., Wheals A. E. 1979; Dependency of size of Saccharomyces cerevisiae cells on growth rate. Journal of Bacteriology 138:92–98
    [Google Scholar]
  27. Unger M. V., Hartwell L. H. 1976; Control of cell division in Saccharomyces cerevisiae by methionyl-tRNA. Proceedings of the National Academy of Sciences of the United States of America 73:1664–1668
    [Google Scholar]
  28. Wolfner M., Yep D., Messenguy F., Fink G. R. 1975; Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae. Journal of Molecular Biology 90:273–290
    [Google Scholar]
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