Transfer of Nitrate Reductase Genes of the Cyanobacterium Nostoc muscorum into Rhizobium japonicum Free

Abstract

Transformation of CB1809 was studied using DNA from the cyanobacterium ATCC 27893. A spontaneous nitrate reductase deficient (Nar) mutant (NR-6) of CB1809 was isolated with a frequency of 8·4 × 10. Streptomycin (Sm) and Neomycin (Neo) resistance markers were introduced into strain NR-6, and the resulting strain was designated NR-6 Sm Neo. Experiments with cyanobacterial DNA and live cells of strain NR-6 Sm Neo indicated transformation of nitrate reductase () genes of into this strain. This conclusion was supported by the reversion frequency of strain NR-6 Sm Neo to Nar and the transformation frequency when recipient cells were exposed to DNA (with heat-treated DNA as control). Comparisons of growth, nitrate uptake, assimilatory nitrate reductase activity and nodulation of parent CB1809, NR-6 Sm Neo and five transformant clones (Nar) suggest that there may be considerable homology between the genes of CB1809 and

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1983-08-01
2024-03-29
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References

  1. Brill W.J. 1980; Biochemical genetics of nitrogen fixation. Microbiological Reviews 44:449–467
    [Google Scholar]
  2. Devilly C.I., Houghton J.A. 1977; A study of genetic transformation in Gloeocapsa alpicola.. Journal of General Microbiology 98:277–280
    [Google Scholar]
  3. Fisher R., Tuli R., Haselkorn R. 1981; A cloned cyanobacterial gene for glutamine synthetase functions in Escherichia coli, but the enzyme is not adenylylated. Proceedings of the National Academy of Sciences of the United States of America 78:3393–3397
    [Google Scholar]
  4. Gerloff G.C., Fitzgerald G.P., Skoog F. 1950; The isolation, purification and culture of blue-green algae. American Journal of Botany 37:216–218
    [Google Scholar]
  5. Guerrero M.G., Vega J.M., Losada M. 1981; The assimilatory nitrate reductase system and its regulation. Annual Review of Plant Physiology 32:169–204
    [Google Scholar]
  6. Herbert D., Phipps P.J., Strange R.E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  7. Ludwig R.A., Signer E.R. 1977; Glutamine synthetase and control of nitrogen fixation in Rhizobium. Nature; London: 267245–247
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  9. Pagan J.D., Scrowcroft W.R., Dudman W.F., Gibson A.H. 1977; Nitrogen fixation in nitrate reductase deficient mutants of cultured rhizobia. Journal of Bacteriology 129:718–723
    [Google Scholar]
  10. Schwinghamer E.A. 1976; Genetic aspects of nodulation and dinitrogen fixation by legumes: the microsymbiont. In A Treatise on Dinitrogen Fixation, Section III pp. 577–622 Hardy R.W.F., Silver W.S. Edited by New York & London: John Wiley.;
    [Google Scholar]
  11. Singh R.K., Singh H.N. 1981; Genetic analysis of het and nif genes in the blue-green alga Nostoc muscorum.. Molecular and General Genetics 184:531–535
    [Google Scholar]
  12. Snell F.D., Snell C.J. 1949 Colorimetric Methods of Analysis II p. 802 Princeton, New Jersey: Van Nostrand;
    [Google Scholar]
  13. Stevens S.E., Porter R.D. 1980; Transformation in Agmenellum quadruplicatum.. Proceedings of the National Academy of Sciences of the United States of America 77:6052–6056
    [Google Scholar]
  14. Vincent J.M. 1970 A Manual for the Practical Study of Root Nodule Bacteria IBP Handbook 15 p. 82 Oxford & Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
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