1887

Abstract

Forespores of 60015 were isolated from sporulating cells at and were incubated with an amino acid mixture containing [C]phenylalanine. Three species of C-labelled cytoplasmic proteins synthesized in the forespores were purified to homogeneity by gel filtration in the presence of detergents and by ion-exchange chromatography. The molecular weights of the purified proteins, called A, B and C, were estimated by SDS-PAGE as 24200, 11500 and 12700, respectively. Protein B contained remarkably high amounts of tyrosine (23·8%) and alanine (22·8%), and proteins A and C contained relatively high amounts of glutamate + glutamine, glycine, and alanine (10·4 to 13·3%). The synthesis of these proteins can provide markers for the control events in the forespore compartment during sporulation.

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1983-08-01
2024-03-28
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References

  1. Ames G.F.-L. 1974; Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Journal of Biological Chemistry 249:634–644
    [Google Scholar]
  2. Andreoli A.J., Suehiro S., Sakiyama D., Takemoto J., Vivanco E., Lara J.C., Klute M.C. 1973; Release and recovery of forespores from Bacillus cereus. Journal of Bacteriology 115:1159–1166
    [Google Scholar]
  3. Andreoli A.J., Saranto J., Baecker P.A., Suehiro S., Escamilla E., Steiner A. 1975; Biochemical properties of forespores isolated from Bacillus cereus. In Spores VI pp. 418–433 Edited by Gerhardt G., Costilow R. N., Sadoff H. L. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  4. Andreoli A.J., Saranto J., Caliri N., Escamilla E., Pina E. 1978; Comparative study of proteins from forespore and mother-cell compartments of Bacillus cereus. In Spores VII pp. 260–264 Edited by Chambliss G., Vary J. C. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  5. Dignam S.S., Setlow P. 1980; In vivo and in vitrosynthesis of the spore-specific proteins A and C of Bacillus megaterium. Journal of Biological Chemistry 255:8417–8423
    [Google Scholar]
  6. Eaton M.W., Ellar D.J. 1974; Protein synthesis and breakdown in the mother-cell and forespore compartments during spore morphogenesis in Bacillus megaterium. Biochemical Journal 144:327–337
    [Google Scholar]
  7. Ellar D.J., Posgate J.A. 1974; Characterization of forespores isolated from Bacillus megaterium at different stages of development into mature spores. In Spore Research 1973 pp. 21–40 Edited by Barker A. N., Gould G. W., Wolf J. London & New York: Academic Press;
    [Google Scholar]
  8. Ellar D.J., Eaton M.W., Hogarth C., Wilkinson B.J., Deans J., Nauze J.L. 1975; Comparative biochemistry and function of forespore and mother-cell compartments during sporulation of Bacillus megaterium cells. In Spores VI pp. 425–433 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  9. Fairbanks G., Steck T.L., Wallach D.F.H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  10. Freese E. 1976; Metabolic control of sporulation. In Spore Research 1976 pp. 1–32 Edited by Barker N., Gould G. W., Wolf J. London & New York: Academic Press;
    [Google Scholar]
  11. Fujita Y., Ramaley R., Freese E. 1977; Location and properties of glucose dehydrogenase in sporulating cells and spores of Bacillus subtilis. Journal of Bacteriology 132:282–293
    [Google Scholar]
  12. Hachisuka Y., Tochikubo K. 1971; Reactivate action of ethylenediaminetetraacetic acid or dipico- linic acid on inactive glucose dehydrogenase obtained from heated spores of Bacillus subtilis. Journal of Bacteriology 107:442–447
    [Google Scholar]
  13. Johnson W.C., Tipper D.J. 1981; Acid-soluble spore proteins of Bacillus subtilis. Journal of Bacteriology 146:972–982
    [Google Scholar]
  14. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Linn T., Losick R. 1976; The program of protein synthesis during sporulation in Bacillus subtilis. Cell 8:103–114
    [Google Scholar]
  16. Nakayama T., Munoz L., Doi R.H. 1977; A procedure to remove protease activities from Bacillus subtilis sporulating cells and their crude extracts. Analytical Biochemistry 78:165–170
    [Google Scholar]
  17. Nakayama T., Kurogi Y., Irikura M., Matsuo H., Sasaki-Wada A. 1980; Fractionation and biochemical properties of the mother cell and forespore fractions from sporulating cells of Bacillus subtilis. Journal of Biochemistry 88:317–326
    [Google Scholar]
  18. Schaeffer P. 1969; Sporulation and the production of antibiotics, exoenzymes, and exotoxins. Bacteriological Review 33:48–71
    [Google Scholar]
  19. Setlow P. 1975a; Identification and localization of the major proteins degraded during germination of Bacillus megaterium spores. Journal of Biological Chemistry 250:8159–8167
    [Google Scholar]
  20. Setlow P. 1975b; Purification and properties of some unique low-molecular-weight basic proteins degraded during germination of Bacillus megateriumspores. Journal of Biological Chemistry 250:8168–8175
    [Google Scholar]
  21. Setlow P. 1978; Purification and characterization of additional low-molecular-weight basic proteins degraded during germination of Bacillus megateriumspores. Journal of Bacteriology 136:331–340
    [Google Scholar]
  22. Setlow P. 1981; Biochemistry of bacterial forespore development and spore germination. In Sporulation and Germination pp. 13–28 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  23. Setlow P., Primus G. 1975; Protein metabolism during germination of Bacillus megaterium spores. I. Protein synthesis and amino acid metabolism. Journal of Biological Chemistry 250:623–630
    [Google Scholar]
  24. Setlow B., Setlow P. 1979; Localization of low- molecular-weight basic proteins in Bacillus megaterium spores by cross-linking with ultraviolet light. Journal of Bacteriology 139:486–494
    [Google Scholar]
  25. Tipper D.J., Johnson W.C., Chambliss G.H., Mahler I., Arnaud M., Halvorson H.O. 1981; Acid-soluble polypeptides of Bacillus subtilisspores. In Sporulation and Germination pp. 178–183 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  26. Watabe K., Iida S., Wada R., Ichikawa T., Kondo M. 1980; Characterization of forespores isolated from Bacillus subtilis at each stage of sporulation. Microbiology and Immunology 24:79–82
    [Google Scholar]
  27. Watabe K., Iida S., Nakamura K., Ichikawa T., Kondo M. 1981; Protein synthesis in the isolated forespores from sporulating cells of Bacillus subtilis. Microbiology and Immunology 25:545–556
    [Google Scholar]
  28. Yuan K., Johnson W.C., Tipper D.J., Setlow P. 1981; Comparison of various properties of low- molecular-weight proteins from dormant spores of several Bacillus species. Journal of Bacteriology 146:965–971
    [Google Scholar]
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