The Effect of Membrane-bound -Lactamase on Linoleic Acid Sensitivity in Free

Abstract

The presence of a plasmid conferring resistance to penicillin (PC plasmid, e.g. pI I) in NCTC 8325 increases the sensitivity of such a bacterium to the growth inhibitory effects of linoleic acid, whereas a plasmid conferring resistance to tetracycline does not affect linoleic acid sensitivity. The increased linoleic acid sensitivity of bacteria containing a PC plasmid may be related to the penicillinase protein itself since (i) strains having inducible penicillinase show increased sensitivity only after induction, (ii) strains in which penicillinase is directed from chromosomal or plasmid-borne genes show similar increased linoleic acid sensitivity and (iii) notwithstanding the above, the linoleic acid inhibitory effect is enhanced in a strain in which penicillinase activity is greatly reduced by a point mutation in the structural gene for penicillinase. The enhanced linoleic acid sensitivity seems to require the membrane-bound penicillinase since added extracellular penicillinase does not confer this sensitivity, and there appears to be a specific interaction between the membrane-bound penicillinase activity and linoleic acid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-8-2457
1983-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/8/mic-129-8-2457.html?itemId=/content/journal/micro/10.1099/00221287-129-8-2457&mimeType=html&fmt=ahah

References

  1. Ambler R.P. 1980; The structure of β-lactamases. Philosophical Transactions of the Royal Society. Series B 289:321–331
    [Google Scholar]
  2. Ambler R.P., Meadway R.J. 1969; Chemical structure of bacterial penicillinases. Nature; London: 22224–26
    [Google Scholar]
  3. Arber A. 1960; Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11:273–288
    [Google Scholar]
  4. Asheshov E.H. 1966; Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcus aureus. Nature; London: 210804–806
    [Google Scholar]
  5. Asheshov E.H. 1969; The genetics of penicillinase production in Staphylococcus aureus strain PS80. Journal of General Microbiology 59:289–301
    [Google Scholar]
  6. Asheshov E. H. 1975; The genetics of tetracycline resistance in Staphylococcus aureus. . Journal of General Microbiology 88:132–140
    [Google Scholar]
  7. Butcher G.W., King G., Dyke K.G.H. 1976; Sensitivity of Staphylococcus aureus to unsaturated fatty acids. Journal of General Microbiology 94:290–296
    [Google Scholar]
  8. Collins J.F. 1971; The regulation of penicillinase synthesis in Gram-positive bacteria. In Metabolic Pathways, 3rd edn.. 5 pp. 489–523 Vogel H.J. Edited by London & New York: Academic Press;
    [Google Scholar]
  9. Freese E. 1959; Difference between spontaneous and base analogue induced mutations of phage T4. Proceedings of the National Academy of Sciences of the United States of America 45:622–633
    [Google Scholar]
  10. Greenway D.L.A., Dyke K.G.H. 1979; Mechanism of the inhibitory action of linoleic acid on the growth of Staphylococcus aureus. . Journal of General Microbiology 115:233–245
    [Google Scholar]
  11. Hayes W. 1968 The Genetics of Bacteria and their Viruses, 2nd edn.. pp. 297–338 Oxford & Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  12. Indue M., Hashimoto M., Mitsuhashi S. 1970; Mechanisms of tetracycline resistance in Staphylococcus aureus. I. Inducible resistance to tetracycline. Journal of Antibiotics 23:68–74
    [Google Scholar]
  13. Johnston L.H., Dyke K.G.H. 1971; Stability of penicillinase plasmids in Staphylococcus aureus. . Journal of Bacteriology 107:63–67
    [Google Scholar]
  14. Lacey R.W. 1973; Genetic basis, epidemiology and future significance of antibiotic resistance in Staphylococcus aureus. . Journal of Clinical Pathology 26:899–913
    [Google Scholar]
  15. Lai J.-S., Sarvas M., Brammar W.J., Neugebauer K. . 1981; Bacillus licheniformispenicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride. Proceedings of the National Academy of Sciences of the United States of America 78:3506–3510
    [Google Scholar]
  16. Leggate J., Holms W.H. 1968; Gratuitous synthesis of β-lactamase in Staphylococcus aureus. . Journal of Bacteriology 96:2110–2117
    [Google Scholar]
  17. Nielsen J.B.K., Lampen J.O. 1982; Membrane-bound penicillinases in Gram-positive bacteria. Journal of Biological Chemistry 257:4490–4495
    [Google Scholar]
  18. Neilsen J.B.K., Caulfield M.P., Lampen J.O. 1981; Lipoprotein nature of Bacillus licheniformismembrane penicillinase. Proceedings of the National Academy of Sciences of the United States of America 78:3511–3515
    [Google Scholar]
  19. Novick R.P. 1962; Staphylococcal penicillinase and new penicillins. Biochemical Journal 83:229–236
    [Google Scholar]
  20. Novick R.P. 1963; Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. . Journal of General Microbiology 33:121–136
    [Google Scholar]
  21. Perret C.J. 1954; Iodometric assay of penicillinase. Nature; London: 1741012–1013
    [Google Scholar]
  22. Richmond M.H. 1963; Purification and properties of the exopenicillinase of Staphylococcus aureus. . Biochemical Journal 88:452–459
    [Google Scholar]
  23. Richmond M.H. 1965; Wild-type variants of exopenicillinases from Staphylococcus aureus. Biochemical Journal 94:584–593
    [Google Scholar]
  24. Robson B., Pain R.H. 1976; Mechanism of folding of globular proteins. Suitability of a penicillinase from Staphylococcus aureus as a model for refolding. Biochemical Journal 155:325–330
    [Google Scholar]
  25. Smith R., Tanford C. 1973; Hydrophobicity of long chain n-alkyl carboxylic acids as measured by their distribution between heptane and aqueous solutions. Proceedings of the National Academy of Sciences of the United States of America 70:289–293
    [Google Scholar]
  26. Smith W.P., Tai P-C., Davis B.D. 1981; Bacillus licheniformis penicillinase: cleavages and attachment of lipid during cotranslational secretion. Proceedings of the National Academy of Sciences of the United States of America 78:3501–3505
    [Google Scholar]
  27. Sompolinsky D., Zaidenzaig Y., Ziegler-Schlomowitz R., Abramova N. 1970; Mechanism of tetracycline resistance in Staphylococcus aureus. . Journal of General Microbiology 62:351–362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-8-2457
Loading
/content/journal/micro/10.1099/00221287-129-8-2457
Loading

Data & Media loading...

Most cited Most Cited RSS feed