1887

Abstract

Using [H]corticosterone as a probe, corticosteroid-binding protein (CBP) was detected in eight out of eight isolates of , of both A and B serotypes. The apparent dissociation constant ( ) in the various isolates ranged between 8 and 19 n; the binding capacity varied from 122 to over 2400 fmol (mg cytosol protein). There was no correlation between the amount or affinity of CBP and isolate virulence for murine hosts. Further analysis revealed demonstrable CBP in six out of six species other than One isolate of has been identified which fails to bind [H]corticosterone. and also failed to bind [H]corticosterone. Preliminary attempts were made to determine functions mediated by CBP in , but growth, phase conversion and glucose oxidation by were unaffected by the addition of a variety of steroid hormones. These data indicate that the presence of CBP in does not correlate with either virulence or serotype. The physiological significance of CBP remains to be determined.

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/content/journal/micro/10.1099/00221287-129-8-2379
1983-08-01
2021-07-29
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References

  1. Barksdale A.W. 1969; Sexual hormones of Achlyaand other fungi. Science 166:831–837
    [Google Scholar]
  2. Betz R., Manney T.R., Duntze W. 1981; Hormonal control of gametogenesis in the yeast Saccharomyces cerevisiae. Gamete Research 4:571–584
    [Google Scholar]
  3. Drouhet E., Balota V., Borderon J.C., Rousset J.J. 1971; Recherches sur les serotypes A et B de C.albicans. Bulletin de la Société francaise de mycologie medicale 19:30–31
    [Google Scholar]
  4. Feldman D., Do Y., Burshell A., Stathis P., Loose D. 1982; An estrogen binding protein and endogenous ligand in the yeast Saccharomyces cerevisiae: a possible steroid-receptor system. Science 218:297–298
    [Google Scholar]
  5. Feldman D., Loose D., Stover P., Restrepo A., Stevens D. 1983; A receptor-like estrogen binding protein in Paracoccidiodes brasilensis (Pb): possible basis for lower infection rate in women than men. Clinical Research 31:470A
    [Google Scholar]
  6. Frenkel J.K. 1962; Role of corticosteroids as predisposing factors in fungal diseases. Laboratory Investigation 11:1192–1208
    [Google Scholar]
  7. Galgiani J.N., Stevens D.A. 1976; Antimicrobial susceptibility testing of yeasts: a turbidi-metric technique independent of inoculum size. Antimicrobial Agents and Chemotherapy 10:721–726
    [Google Scholar]
  8. Gooday G.W. 1974; Fungal sex hormones. Annual Review of Biochemistry 43:35–49
    [Google Scholar]
  9. Hurley R. 1980; The pathogenic Candida species and diseases caused by Candida in man. In Biology and Activities of Yeasts pp. 231–248 Edited by Skinner F. A., Passmore S. M., Davenport R. R. New York: Academic Press;
    [Google Scholar]
  10. Lehrer R.I. 1970; Measurement of candidacidal activity of specific leukocyte types in mixed cell populations. I. Normal, myeloperoxidase-deficient, and chronic granulomatous disease neutrophils. Infection and Immunity 2:42–47
    [Google Scholar]
  11. Loose D.S., Feldman D. 1982; Characterization of a unique corticosterone-binding protein in Candida albicans. Journal of Biological Chemistry 257:4925–4930
    [Google Scholar]
  12. Loose D.S., Schurman D.J., Feldman D. 1981; A corticosteroid binding protein and endogenous ligand in C.albicans indicating a possible steroid-receptor system. Nature, London 293:477–479
    [Google Scholar]
  13. Mariat F. 1964; Saprophytic and parasitic morphology of pathogenic fungi. In Microbial Behaviour In Vivo and In Vitro pp. 85–111 Edited by Smith H., Taylor J. Cambridge: University Press;
    [Google Scholar]
  14. Munck A., Zyskowski L. 1975; Methods for assessing kinetics of hormone effects on energy and transport mechanisms in cells in suspension. Methods in Enzymology 36A:429–433
    [Google Scholar]
  15. Pena C. 1967; Deep mycotic infections in Colombia: a clinicopathologic study of 162 cases. American Journal of Clinical Pathology 47:505–520
    [Google Scholar]
  16. Scatchard G. 1949; The attractions of proteins for small molecules and ions. Annals of the New York Academy of Sciences 51:660–672
    [Google Scholar]
  17. Silva-Hutner M., Cooper B.H. 1980; Yeasts of medical importance. In Manual of Clinical Microbiology, 3rd edn. pp. 562–576 Edited by Lennette E. H. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  18. Stiller R.L., Bennett J.E., Scholer H.J., Wall M., Polak A., Stevens D.A. 1982; Susceptibility to 5-fiuorocytosine and prevalence of serotype in 402 Candida albicans isolates from the United States. Antimicrobial Agents and Chemotherapy 22:482–487
    [Google Scholar]
  19. Vanuden N., Buckley H. 1970; Candida Berkhout. In The Yeasts-A Taxonomic Study, 2nd edn. pp. 893–1087 Edited by Lodder J. Amsterdam: North Holland;
    [Google Scholar]
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