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The membrane lipids from an archaebacterial haloalkaliphile were shown to be based almost entirely on diethers containing C25 isopranyl chains. The ‘universal’ C20,C20 archaebacterial diether core lipid (2,3-di-O-phytanyl-sn-glycerol) made up only 9% (w/w) of the total isopranoid diether fraction. The rest of the isopranoid diether fraction comprised 85% (w/w) C20,C25 diether (2-O-sesterterpanyl-3-O-phytanyl-sn-glycerol) and 6% (w/w) of a novel C25,C25 diether (2,3-di-O-sesterterpanyl-sn-glycerol).
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