1887

Abstract

The haemolytic plasmid pSU316 is incompatible with members of the IncFIII and IncFIV incompatibility groups. Plasmid pSU307 (pSU316 :: Tn) was inserted by integrative suppression into the chromosome of JW112, a temperature-sensitive mutant of Escherichia coli. The incompatibility properties of this strain (SU51) were studied and it was found that: (1) plasmid pSU306 (pSU316 :: Tn) was rapidly lost from strain SU51 both at 30 °C and 42 °C; (2) the IncFIII plasmid pSU397 (ColB-K98 :: Tn) was lost from strain SU51 at 42 °C but not at 30 °C; and (3) the IncFIV plasmid R124 was stably maintained in strain SU51 at both temperatures. Revertants of pSU307 to the autonomous state could be obtained from SU51. These revertants exerted incompatibility towards the prototype plasmids pSU306, pSU397 and R124 in the same way as pSU307 itself. Thus, strain SU51 provided a suitable method for distinguishing the three different incompatibility determinants of plasmid pSU316.

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/content/journal/micro/10.1099/00221287-129-7-2277
1983-07-01
2022-01-23
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References

  1. Bennett P. M., Richmond M. H. 1976; Translocation of a discrete piece of DNA carrying an amp gene between replicons in Escherichia coli. Journal of Bacteriology 126:1–6
    [Google Scholar]
  2. Bergquist P. L., Lane H. E. D., Malcolm L., Downard R. A. 1982; Molecular homology and incompatibility in the IncFI plasmid group. Journal of General Microbiology 128:223–238
    [Google Scholar]
  3. Bukhari A. I., Kamp D. 1980; Genetic rearrangements and DNA cleavage maps. Methods in Enzymology 65:436–449
    [Google Scholar]
  4. Chandler M., Silver L., Caro L. 1977; Suppression of an Escherichia coli dnaA mutation by the integrated R factor R 100.1: origin of chromosome replication during exponential growth. Journal of Bacteriology 131:421–430
    [Google Scholar]
  5. De La Cruz F., Zabala J. C., Ortiz J. M. 1979; Incompatibility among α-hemolytic plasmids studied after inactivation of the α-hemolysin gene by transposition of Tn802. Plasmid 2:507–519
    [Google Scholar]
  6. De La Cruz F., Zabala J. C., Ortiz J. M. 1980a; The molecular relatedness among α-hemolytic plasmids from various incompatibility groups. Plasmid 4:76–81
    [Google Scholar]
  7. De La Cruz F., Müller D., Ortiz J. M., Goebel W. 1980b; Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups. Journal of Bacteriology 143:825–833
    [Google Scholar]
  8. Datta N. 1979; Plasmid classification: incompatibility grouping. In Plasmids of Medical, Environmental and Commercial Importance pp. 3–12 Timmis K. N., Pühler A. Edited by Amsterdam: Elsevier-North Holland Biomedical Press;
    [Google Scholar]
  9. Frydman A., Meynell E. 1969; Interaction between derepressed F-like R-factors and wild type colicin B factors: superinfection immunity and repressor susceptibility. Genetical Research 14:315–332
    [Google Scholar]
  10. Gasson M. J., Willetts N. S. 1975; Five control systems preventing transfer of Escherichia coli K-12 sex factor F. Journal of Bacteriology 122:518–525
    [Google Scholar]
  11. Goebel W., Hedgpeth J. 1982; Cloning and functional characterization of the plasmid-encoded hemolysin determinant of E. coli. Journal of Bacteriology 151:1290–1298
    [Google Scholar]
  12. Hedges R. W., Datta N. 1972; R124 an fi+ R factor of a new compatibility class. Journal of General Microbiology 71:403–405
    [Google Scholar]
  13. Kitts P. A., Lamond A., Sherratt D. J. 1982; Inter-replicon transposition of Tn1/3 occurs in two sequential genetically separable steps. Nature; London: 295626–628
    [Google Scholar]
  14. Lane H. E. D. 1981; Replication and incompatibility of F and plasmids in the IncFI group. Plasmid 5:100–126
    [Google Scholar]
  15. Molin S., Nordström K. 1980; Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication. Journal of Bacteriology 141:111–120
    [Google Scholar]
  16. Nishimura Y., Caro L., Berg C. M., Hirota Y. 1971; Chromosome replication in Escherichia coli. IV.Control of chromosome replication and cell division by an integrated episome. Journal of Molecular Biology 55:441–456
    [Google Scholar]
  17. Nordström K., Molin S., Aagaard-Hansen H. 1980; Partitioning of plasmid R1 in Escherichia coli. II. Incompatibility properties of the partitioning system. Plasmid 4:332–349
    [Google Scholar]
  18. Novick R. P., Hoppensteadt F. C. 1978; On plasmid incompatibility. Plasmid 1:421–434
    [Google Scholar]
  19. Sasakawa C., Takamatsu N., Danbara H., Yoshikawa M. 1980; A method of plasmid classification by integrative incompatibility. Plasmid 3:116–127
    [Google Scholar]
  20. Săsărman A., Massie B., Zollinger M., Gagne-Tellier H., Shareck F., Garzon S., Morisset R. 1980; Naturally occurring R.ColBM plasmids belonging to the IncFIII incompatibility group. Journal of General Microbiology 119:475–483
    [Google Scholar]
  21. Seelke R. W., Kline B. C., Trawick J. D., Ritts G. D. 1982; Genetic studies of F plasmid maintenance genes involved in copy number control, incompatibility and partitioning. Plasmid 7:163–179
    [Google Scholar]
  22. Smith H. R., Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Interactions of group H resistance factors with the F factor. Journal of Bacteriology 115:623–628
    [Google Scholar]
  23. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  24. Timmis K. N., Danbara H., Brady G., Lurz R. 1981; Inheritance functions of group IncFII transmissible antibiotic resistance plasmids. Plasmid 5:53–75
    [Google Scholar]
  25. Wechsler J. A., Gross J. D. 1971; Escherichia coli mutants temperature-sensitive for DNA synthesis. Molecular and General Genetics 113:273–284
    [Google Scholar]
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