1887

Abstract

The gene of was inserted into prophage 𝜙105. The recombinant phage (𝜙105d) contained DNA which was about 2 MDal smaller than the wild-type phage DNA, and the phage particles had no tails. The phage did not plaque but, when provided with tails , it transduced both and strains of to . The gene was located on a 2·5 MDal RI restriction fragment. Subsequently this phage was used to clone, using a similar technique, the gene(s). The second recombinant phage, 𝜙105d, was also defective, i.e. without tails. The DNA was 1.5 MDal smaller than the wild-type phage DNA and the gene was located on a 3 MDal RI fragment. When provided with tails , phage 𝜙105d transduced cells of a recipient to Spo. In these transductants the mutation was complemented, and the cells sporulated normally.

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1983-07-01
2021-05-14
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