1887

Abstract

The gene of was inserted into prophage 𝜙105. The recombinant phage (𝜙105d) contained DNA which was about 2 MDal smaller than the wild-type phage DNA, and the phage particles had no tails. The phage did not plaque but, when provided with tails , it transduced both and strains of to . The gene was located on a 2·5 MDal RI restriction fragment. Subsequently this phage was used to clone, using a similar technique, the gene(s). The second recombinant phage, 𝜙105d, was also defective, i.e. without tails. The DNA was 1.5 MDal smaller than the wild-type phage DNA and the gene was located on a 3 MDal RI fragment. When provided with tails , phage 𝜙105d transduced cells of a recipient to Spo. In these transductants the mutation was complemented, and the cells sporulated normally.

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1983-07-01
2024-12-06
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References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Birdsell D.C., Hathaway G.M., Rutberg L. 1969; Characterization of temperate Bacillusbacteriophage ø105. Journal of Virology 4:264–270
    [Google Scholar]
  3. Coote J.G. 1972; Sporulation in Bacillus subtilis.Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  4. Dancer B.N., Mandelstam J. 1981; Complementation of sporulation mutations in fused protoplasts of Bacillus subtilis. Journal of General Microbiology 123:17–26
    [Google Scholar]
  5. Dubnau D., Davidoff-Abelson R., Scher B., Cirigliano C. 1973; Fate of transforming deoxy-ribonucleic acid after uptake by competent Bacillus subtilis: phenotypic characterization of radiation- sensitive recombination-deficient mutants. Journal of Bacteriology 114:273–286
    [Google Scholar]
  6. Flock J., Rutberg L. 1974; Mature DNA from temperate Bacillus phage 0105 requires primary recombination to be infectious in transfection. Molecular and General Genetics 131:301–311
    [Google Scholar]
  7. Glenn A.R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  8. Hirochika H., Kobayashi Y., Kawamura F., Saito H. 1981; Cloning of sporulation gene spoOBof Bacillus subtilis and its genetic and biochemical analysis. Journal of Bacteriology 146:494–505
    [Google Scholar]
  9. Hoch J.A. 1971; Selection of cells transformed to prototrophy for sporulation markers. Journal of Bacteriology 105:1200–1201
    [Google Scholar]
  10. Hranueli D., Piggot P.J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  11. Iijima T., Kawamura F., Saito H., Ikeda Y. 1980; A specialized transducing phage constructed from Bacillus subtilis phage ø105. Gene 9:115–126
    [Google Scholar]
  12. Jenkinson H.F. 1983; Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis. Journal of General Microbiology 129:1945–1958
    [Google Scholar]
  13. Jenkinson H.F., Kay D., Mandelstam J. 1980; Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  14. Kawamura F., Saito H., Hirochika H., Kobayashi Y. 1980; Cloning of sporulation gene, spoOF,in Bacillus subtilis with p11 phage vector. Journal of General and Applied Microbiology 26:345–355
    [Google Scholar]
  15. Kawamura F., Shimotsu H., Saito H., Hirochika H., Kobayashi Y. 1981; Cloning of spoO genes with bacteriophage and plasmid vectors in Bacillus subtilis. In Sporulation and Germination pp. 109–113 Levinson H.S., Sonenshein A.L., Tipper D.L. Edited by Washington, D.C.:: American Society for Microbiology.;
    [Google Scholar]
  16. Lencastre H.D., Piggot P.J. 1979; Identification of different sites of expression for spo loci by transformation of Bacillus subtilis. Journal of General Microbiology 114:377–389
    [Google Scholar]
  17. Liu H-M., Chak K.F., Piggot P.J. 1982; Isolation and characterization of a recombinant plasmid carrying a functional part of the Bacillus subtilis spoil A locus. Journal of General Microbiology 128:2805–2812
    [Google Scholar]
  18. Lloyd-Jones G. 1976 Genetics and biochemistry of bacterial sporulation. M.Sc. thesis University of Oxford, U.K.:
    [Google Scholar]
  19. Losick R., Pero J. 1981; Cascades of sigma factors. Cell 25:582–584
    [Google Scholar]
  20. Mazza G., Galizzi A. 1978; The genetics of DNA replication, repair and recombination in Bacillus subtilis. Microbiologica 1:111–135
    [Google Scholar]
  21. Moran C.P.Jr Lang N., Legrice S.F.J., Lee G., Stephens M., Sonenshein A.L., Pero J., Losick R. 1982; Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Molecular and General Genetics 186:339–346
    [Google Scholar]
  22. Nester E.W., Schafer M., Lederberg J. 1963; Gene linkage in DNA transfer: a cluster of genes concerned with aromatic biosynthesis in Bacillus subtilis. Genetics 48:529–551
    [Google Scholar]
  23. Okamoto K., Mudd J.A., Mangan J., Huang W.M., Subbaiah T.V., Marmur J. 1968a; Properties of the defective phage of Bacillus subtilis. Journal of Molecular Biology 34:413–428
    [Google Scholar]
  24. Okamoto J., Mudd J.A., Marmur J. 1968b; Conversion of Bacillus subtilis DNA to phage DNA following mitomycin C induction. Journal of Molecular Biology 34:429–437
    [Google Scholar]
  25. Perkins J.B., Zarley C.D., Dean D.H. 1978; Restriction endonuclease mapping of bacteriophage ø105 and closely related temperate Bacillus subtilisbacteriophages p10 and p14. Journal of Virology 28:403–407
    [Google Scholar]
  26. Piggot P.J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  27. Piggot P.J., Coote J.G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  28. Piggot P.J., Moir A., Smith D.A. 1981; Advances in the genetics of Bacillus subtilisdifferentiation. In Sporulation and Germination pp. 29–39 Levinson H.S., Sonenshein A.L., Tipper D.J. Edited by Washington, D.C.:: American Society for Microbiology.;
    [Google Scholar]
  29. Sadoff H.L. 1966; Glucose dehydrogenase-soluble. I.Bacillus cereus. Methods in Enzymology 9:103–107
    [Google Scholar]
  30. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Colloques intemationaux du Centre national de la recherche scientifique 124:553–563
    [Google Scholar]
  31. Scher B.M., Law M.F., Garro A.J. 1978; Correlated genetic and EcoRI cleavage map of Bacillus subtilis bacteriophage ø105 DNA. Journal of Virology 28:395–402
    [Google Scholar]
  32. Sterlini J.M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  33. Ward J.B.Jr Zahler S.A. 1973; Genetic studies of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:719–726
    [Google Scholar]
  34. Yamamoto K.R., Alberts B.M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
  35. Young M., Mandelstam J. 1979; Early events during bacterial endospore formation. Advances in Microbial Physiology 20:103–162
    [Google Scholar]
  36. Yudkin M.D., Turley L. 1981; Mapping of six mutations in the spoII A locus of Bacillus subtilis and studies of their response to a nonsense suppressor. Journal of General Microbiology 124:255–261
    [Google Scholar]
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