1887

Abstract

The serological properties of cell surface proteins of belonging to both the biotypes (classical and El Tor) and the serotypes (Ogawa and Inaba) were investigated. Proteins were isolated by extracting with EDTA in the presence of sodium chloride. The surface localization of these proteins was confirmed with (a) radioiodinated protein A as an immunoprobe and (b) antiserum absorption studies with whole bacteria. There were similarities among the polypeptides of cell surface proteins isolated from various types. Antisera to these proteins agglutinated several strains, irrespective of biotype, serotype and antibiotic sensitivity. The antisera did not agglutinate pathogenic enteric bacteria such as enterotoxigenic and The cell surface proteins of were immunogenic in rabbits as high titres of anti-protein specific antibodies were detected by the ELISA technique in the immune sera. These results suggest that the cell surface proteins are common antigens of and can be developed as a potential vaccine candidate against cholera.

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/content/journal/micro/10.1099/00221287-129-7-2199
1983-07-01
2021-10-26
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References

  1. Bhattacharyya F.K. 1977; The agglutination reaction of cholera vibrios. Japan Journal of Medical Science and Biology 30:259–268
    [Google Scholar]
  2. Craig J.P. 1980; A survey of the enterotoxicenteropathies. In Cholera and Related Diarrheas pp. 15–25 Edited by Ouchterlony Ö., Holmgren J. Basel: Krager;
    [Google Scholar]
  3. Crumpton M.J., Wilkinson J.M. 1963; Amino acid compositions of human and rabbit γ-globulins and of the fragments produced by reduction. Biochemical Journal 88:228–234
    [Google Scholar]
  4. Field M. 1971; Intestinal secretion: effect of cyclic AMP and its role in cholera. New England Journal of Medicine 284:1137–1144
    [Google Scholar]
  5. Geyer R., Galanos C., Westphal O., Lüderitz O. 1979; A lipopolysaccharide-binding cell surface protein from Salmonella minnesota. European Journal of Biochemistry 98:27–38
    [Google Scholar]
  6. Hunter W.M., Greenwood F.C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  7. Kabir S. 1980; Composition and immunochemical properties of the outer membrane proteins of Vibrio cholerae. Journal of Bacteriology 144:382–389
    [Google Scholar]
  8. Kabir S., Mann P. 1980; Immunological properties of the cell envelope components of Vibrio cholerae. Journal of General Microbiology 119:517–525
    [Google Scholar]
  9. King J., Laemmli U.K. 1971; Polypeptides of the tail fibres of bacteriophage T4. Journal of Molecular Biology 62:465–477
    [Google Scholar]
  10. Langone J.J., Boyle D.P.M., Borsos T. 1977; 12SI-Protein A: applications to the quantitative determination of fluid phase and cell-bound IgG. Journal of Immunological Methods 18:281–293
    [Google Scholar]
  11. Levine M.M., Nalin D.R., Craig J.P., Hoover D., Bergquist E.J., Waterman D., Holley H.P., Hornick R.B., Pierce N.F., Libonati J.P. 1979; Immunity to cholera in man: relative role of antibacterial versus antitoxic immunity. Transactions of the Royal Society of Tropical Medicine and Hygiene 73:3–9
    [Google Scholar]
  12. Majumdar A.S., Dutta P., Dutta D., Ghose A.C. 1981; Antibacterial and antitoxin responses in the serum and milk of cholera patients. Infection and Immunity 32:1–8
    [Google Scholar]
  13. Sjoquist J., Meloun B., Helm H. 1972; Protein A isolated from Staphylococcus aureus after digestion with lysostaphin. European Journal of Biochemistry 29:572–578
    [Google Scholar]
  14. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Methods in Carbohydrate Chemistry 5:83–96
    [Google Scholar]
  15. Westphal O., Lüderitz O., Bister F. 1952; Über die Extraktion von Bakterienmit Phenol-wasser. Zeitschrift fur Naturforschung B7:148–155
    [Google Scholar]
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