@article{mbs:/content/journal/micro/10.1099/00221287-129-7-2143, author = "Salmon, Ian and Poole, Robert K.", title = "The Cell Cycle of the Budding Yeast Sterigmatomyces halophilus: Oscillations in the Amounts and Activities of the Terminal Components of the Respiratory Chain", journal= "Microbiology", year = "1983", volume = "129", number = "7", pages = "2143-2152", doi = "https://doi.org/10.1099/00221287-129-7-2143", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-7-2143", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Cells harvested from exponentially growing cultures of the budding yeast Sterigmatomyces halophilus were fractionated according to their age in the cell cycle by isopycnic-zonal centrifugation in Urografin gradients. Activities of five enzymes were assayed in cell-free extracts, prepared using a French pressure cell. The activities of catalase and three enzymes of the mitochondrial inner membrane (NADH dehydrogenase, succinate dehydrogenase and cytochrome c oxidase) doubled during the cell cycle but showed complex oscillatory patterns. Acid phosphatase activity increased continuously during the cell cycle. Fourth-order finite difference analysis of low-temperature difference spectra showed that the levels of three b-type cytochromes increased continuously during the cycle. In contrast, amounts of cytochromes c 1, c and aa 3 oscillated over one cycle, with cytochromes c and aa 3 rising to two maxima per cycle in phase with cytochrome c oxidase activity. Liganding of redox-active metals, and discontinuous synthesis and proteolysis of apocytochromes are discussed as possible mechanisms for cell cycle-dependent fluctuations in the composition and function of the respiratory chain.", }