1887

Abstract

Cells harvested from exponentially growing cultures of the budding yeast were fractionated according to their age in the cell cycle by isopycnic-zonal centrifugation in Urografin gradients. Activities of five enzymes were assayed in cell-free extracts, prepared using a French pressure cell. The activities of catalase and three enzymes of the mitochondrial inner membrane (NADH dehydrogenase, succinate dehydrogenase and cytochrome oxidase) doubled during the cell cycle but showed complex oscillatory patterns. Acid phosphatase activity increased continuously during the cell cycle. Fourth-order finite difference analysis of low-temperature difference spectra showed that the levels of three -type cytochromes increased continuously during the cycle. In contrast, amounts of cytochromes , and oscillated over one cycle, with cytochromes and rising to two maxima per cycle in phase with cytochrome oxidase activity. Liganding of redox-active metals, and discontinuous synthesis and proteolysis of apocytochromes are discussed as possible mechanisms for cell cycle-dependent fluctuations in the composition and function of the respiratory chain.

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1983-07-01
2021-10-22
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References

  1. Aldridge J., Pye E.K. 1976; Cell density dependence of oscillatory metabolism. Nature, London 259:670–671
    [Google Scholar]
  2. Arrigoni O., Singer T.P. 1962; Limitations of the phenazine methosulphate assay for succinic acid and related dehydrogenases. Nature, London 193:1256–1258
    [Google Scholar]
  3. Bakalkin G.Y., Kalnov S.L., Galkin A.V., Zubatov A.S., Luzikov V.N. 1978; The lability of the products of mitochondrial protein synthesis in Saccharomyces cerevisiae A novel method for protein half-life determination. Biochemical Journal 170:569–576
    [Google Scholar]
  4. Cottrell S.F., Rabinowitz M., Getz G.S. 1975; Cytochrome synthesis in synchronous cultures of the yeast, Saccharomyces cerevisiae. Journal of Biological Chemistry, 250:4087–4094
    [Google Scholar]
  5. Duque-MagalhĀes M.C., Ferreira M.M.M. 1980; Cytochrome c degrading activity in rat liver mitochondria. Biochemical and Biophysical Research Communications 93:106–112
    [Google Scholar]
  6. Edwards S.W., Evans J.B., Lloyd D. 1981; Oscillatory accumulation of catalase during the cell cycle of Acanthamoeba castellanii. Journal of General Microbiology 125:459–462
    [Google Scholar]
  7. Elliott S.G., Mclaughlin C.S. 1978; Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 75:4384–4388
    [Google Scholar]
  8. Galkin A.V., Tsoi T.V., Luzikov V.N. 1979; Regulation of mitochondrial biogenesis: further evidence for proteinase involvement. FEBS Letters 105:373–375
    [Google Scholar]
  9. Gear A.R.L., Albert A.D., Bedwarek J.M. 1974; The effect of the hypocholesterolemic drug clofibrate on liver mitochondrial biogenesis. A role for neutral mitochondrial proteases. Journal of Biological Chemistry 249:6495–6504
    [Google Scholar]
  10. Halvorson H.O. 1977; The cell division cycle. In Cell Differentiation in Microorganisms, Plants and Animals pp. 361–376 Edited by Nover L., Mothes K. Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  11. Hare J.F. 1978; A novel proteinase associated with mitochondrial membranes. Biochemical and Biophysical Research Communications 83:1206–1215
    [Google Scholar]
  12. Hennig B., Neupert W. 1981; Assembly of cytochrome c. Apocytochrome c is bound to specific sites on mitochondria before its conversion to holocytochrome c. European Journal of Biochemistry 121:203–212
    [Google Scholar]
  13. Von Jagow G., Weiss H., Klingenberg M. 1973; Comparison of the respiratory chain of Neurospora crassa wild-type and the mi-mutants mi-1 and mi-3. European Journal of Biochemistry 33:140–157
    [Google Scholar]
  14. Joergensen L. 1981; Identification of mitochondrial cytochromes of Tubifex sp. using fourth-order finite difference analysis of low temperature spectra. Comparative Biochemistry and Physiology 69B:769–774
    [Google Scholar]
  15. Keyhani E., Chance B. 1971; Cytochrome biosynthesis under copper-limited conditions in Candida utilis. FEBS Letters 17:127–132
    [Google Scholar]
  16. Keyhani E., Keyhani J. 1980; Identification of porphyrin present in apo-cytochrome c oxidase of copper-deficient yeast cells. Biochimica et biophysica acta 633:211–227
    [Google Scholar]
  17. Kumar C.C., Padmanaban G. 1981; Evidence for the involvement of a rapidly turning-over protease in the degradation of cytochrome oxidase in Neurospora crassa. Biochemical and Biophysical Research Communications 100:576–583
    [Google Scholar]
  18. Lloyd D., Cartledge T.G. 1974; Marker enzymes of eukaryotic micro-organisms. In Methodological Developments in Biochemistry, Vol. 4, Subcellular Studies pp. 339–346 Edited by Reid E. London: Longman;
    [Google Scholar]
  19. Lloyd D., Poole R.K., Edwards S.W. 1982a The Cell Division Cycle: Temporal Organization and Control of Cellular Growth and Reproduction. London: Academic Press;
    [Google Scholar]
  20. Lloyd D., Edwards S.W., Fry J.C. 1982b; Temperature-compensated oscillations in respiration and cellular protein content in synchronous cultures of Acanthamoeba castellanii. Proceedings of the National Academy of Sciences of the United States of America 79:3785–3788
    [Google Scholar]
  21. Lück H. 1963; Catalase. In Methods in Enzyme Analysis pp. 885–894 Edited by Bergmeyer H. U. New York: Academic Press;
    [Google Scholar]
  22. Luzikov V.N., Makhlis T.A., Galkin A.V. 1976; A probable role of cell proteinases in the biogenesis of mitochondria in yeast. FEBS Letters 69:108–110
    [Google Scholar]
  23. Mahler H.R. 1955; DPNH cytochrome c reductase (animal). Methods in Enzymology 2:688–693
    [Google Scholar]
  24. Milner H.W., Lawrence N.S., French C.S. 1950; Colloidal dispersion of chloroplast material. Science 111:633–634
    [Google Scholar]
  25. Morrey J.R. 1968; On determining spectral peak positions from composite spectra with a digital computer. Analytical Chemistry 40:905–914
    [Google Scholar]
  26. Poole R.K., Lloyd D. 1973; Oscillations of enzyme activities during the cell cycle of a glucose-repressed fission yeast Schizosaccharomyces pombe972 h. Biochemical Journal 136:195–207
    [Google Scholar]
  27. Poole R.K., Lloyd D. 1974; Changes in respiratory activities during the cell cycle of the fission yeast Schizosaccharomyces pombe 972 h growing in the presence of glycerol. Biochemical Journal 144:141–148
    [Google Scholar]
  28. Poole R.K., Lloyd D., Chance B. 1974; The development of cytochromes during the cell cycle of a glucose-repressed fission yeast Schizosaccharomyces pombe 972 h. Biochemical Journal 138:201–210
    [Google Scholar]
  29. Rajwade M.S., Katyare S.S., Fatterpaker P., Sreenivasan A. 1975; Regulation of mitochondrial protein turnover by thyroid hormone(s). Biochemical Journal 152:379–387
    [Google Scholar]
  30. Rej R., Richards A.H. 1974; Interference by Tris buffer in the estimation of protein by the Lowry procedure. Analytical Biochemistry 62:240–247
    [Google Scholar]
  31. Richards L., Thurston C.F. 1980; Protein turnover in Chlorella fusca var. vacuolata: measurement of the overall rate of intracellular protein degradation using isotope exchange with water. Journal of General Microbiology 121:49–61
    [Google Scholar]
  32. Roberts D.MCL. 1980; Aspects of cell size measurement in Tetrahymena elliotti. Journal of General Microbiology 120:211–218
    [Google Scholar]
  33. Salmon I., Poole R.K. 1980a; The mitochondrial cytochromes of an unusual budding yeast, Sterigmatomyces halophilus: spectral characterization exploiting fourth-order finite difference analysis. Journal of General Microbiology 117:315–326
    [Google Scholar]
  34. Salmon I., Poole R.K. 1980b; Activities of some mitochondrial enzymes in the cell cycle of Sterigmatomyces halophilus. Society for General Microbiology Quarterly 7:85
    [Google Scholar]
  35. Salmon I., Poole R.K. 1983; The cell cycle of the budding yeast Sterigmatomyces halophilus: culture fractionation by zonal centrifugation and the accumulation of DNA, RNA and protein. Journal of General Microbiology 129:2129–2141
    [Google Scholar]
  36. Singer T.P., Kearney E.B., Kenney W.C. 1973; Succinate dehydrogenase. Advances in Enzymology 37:189–272
    [Google Scholar]
  37. Smith L. 1955; Cytochromes a, a1, a2 and a3. Methods in Enzymology 2:732–740
    [Google Scholar]
  38. Thurston C.F., Richards L. 1980; Protein turnover in Chlorella fusca var.vacuolata : detection of a rapidly degraded class of protein in growing and non-growing cells. Journal of General Microbiology 121:63–68
    [Google Scholar]
  39. Torriani A. 1960; Influence of inorganic phosphate in the formation of phosphatase by Escherichia coli. Biochimica et biophysica acta 38:460–469
    [Google Scholar]
  40. Werner S., Schwab A.J., Neupert W. 1974; Precursors of cytochrome oxidase in cytochrome-oxidase-deficient cells of Neurospora crassa Comparison of the nuclear mutant cni-1, the cytoplasmic mutant mi-1 and copper-depleted wild-type. European Journal of Biochemistry 49:607–617
    [Google Scholar]
  41. Winter D.B., Bruyninckx W.S., Foulke F.G., Grinich N.P., Mason H.S. 1980; Location of heme a on subunits I and II and copper on subunit II of cytochrome c oxidase. Journal of Biological Chemistry 255:11408–11414
    [Google Scholar]
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