%0 Journal Article %A Beeching, John R. %A Weightman, Andrew J. %A Slater, J. Howard %T The Formation of an R-prime Carrying the Fraction I Dehalogenase Gene from Pseudomonas putida PP3 using the IncP Plasmid R64.44 %D 1983 %J Microbiology, %V 129 %N 7 %P 2071-2078 %@ 1465-2080 %R https://doi.org/10.1099/00221287-129-7-2071 %I Microbiology Society, %X Plasmid R68.44 was transferred to Pseudomonas putida PP3 at a frequency of approximately 10−6. In the new strain, P. putida PPW1, the plasmid, designated pUU1, lost a 2·0 kb fragment corresponding to IS21 which had previously been implicated in enhanced chromosome mobilization, but gained between 3·4 and 5·7 kb of DNA. Plasmid pUU1 was used to mobilize the fraction I dehalogenase gene at a low frequency of approximately 10−1 into another strain of P. putida. The R-prime plasmid, designated pUU2, contained an additional 9·2 to 11·0kb fragment which carried the fraction I dehalogenase. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-7-2071