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Plasmid R68.44 was transferred to Pseudomonas putida PP3 at a frequency of approximately 10−6. In the new strain, P. putida PPW1, the plasmid, designated pUU1, lost a 2·0 kb fragment corresponding to IS21 which had previously been implicated in enhanced chromosome mobilization, but gained between 3·4 and 5·7 kb of DNA. Plasmid pUU1 was used to mobilize the fraction I dehalogenase gene at a low frequency of approximately 10−1 into another strain of P. putida. The R-prime plasmid, designated pUU2, contained an additional 9·2 to 11·0kb fragment which carried the fraction I dehalogenase.
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