1887

Abstract

This work describes isolation and characterization of mutants resistant to the lysine analogue -(2-aminoethyl)--cysteine (AEC). The mutation to AEC resistance was shown to affect the feedback regulation of aspartokinase; 70% of the mutants isolated had aspartokinase activity insensitive to concerted feedback inhibition by lysine plus threonine. Among these mutants, 70% (about 50% of the total AEC-resistant strains isolated) showed significant overproduction of -lactam antibiotics.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-7-2063
1983-07-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/7/mic-129-7-2063.html?itemId=/content/journal/micro/10.1099/00221287-129-7-2063&mimeType=html&fmt=ahah

References

  1. Aharonowitz Y., Demain A.L. 1977; Influence of inorganic phosphate and organic buffers on cephalosporin production by Streptomyces clavuligerus. Archives of Microbiology 115:169–173
    [Google Scholar]
  2. Demain A.L. 1957; Inhibition of penicillin formation by lysine. Archives of Biochemistry and Biophysics 67:244–245
    [Google Scholar]
  3. Drew S.W., Demain A.L. 1977; Effect of primary metabolism on secondary metabolism. Annual Review of Microbiology 31:343–356
    [Google Scholar]
  4. Gross W., Burkhardt K.L. 1973; Multiple transport systems for basic amino acid transport in Streptomyces hydrogenans. Biochimica et biophysica acta 298:437–445
    [Google Scholar]
  5. Higgens C.E., Kastner R.E. 1971; Streptomyces clavuligerus sp. nov., a β-lactam antibiotic producer. International Journal of Systematic Bacteriology 21:326–331
    [Google Scholar]
  6. Kern B.A., Hendlin D., Inamine E. 1980; L-lysine µ-aminotransferase involved in cephamycin C synthesis in Streptomyces lactamdurans. Antimicrobial Agents and Chemotherapy 17:679–685
    [Google Scholar]
  7. Kirkpatrick J.R., Doolin L.E., Godfrey O.W. 1973; Lysine biosynthesis in Streptomyces lipmanii: implication in antibiotic biosynthesis. Antimicrobial Agents and Chemotherapy 4:542–550
    [Google Scholar]
  8. Kohsaka M., Demain A.L. 1976; Conversion of penicillin N to cephalosporin(s) by cell free extracts of Cephalosporium acremonium. Biochemical and Biophysical Research Communications 70:465–473
    [Google Scholar]
  9. Luengo J.M., Revilla G., Lopez M.J., Villanueva J.R., Martin J.F. 1980; Inhibition and repression of homocitrate synthase by lysine in Penicillium chrysogenum. Journal of Bacteriology 144:869–876
    [Google Scholar]
  10. Masurekar P.S., Demain A.L. 1972; Lysine control of penicillin biosynthesis. Canadian Journal of Microbiology 18:1045–1048
    [Google Scholar]
  11. Masurekar P.S., Demain A.L. 1974; Impaired penicillin production in lysine regulatory mutants of Penicillium chrysogenum. Antimicrobial Agents and Chemotherapy 6:366–368
    [Google Scholar]
  12. Mendelovitz S., Aharonowitz Y. 1982; Regulation of cephamycin C synthesis, aspartokinase, dihydropicolinic acid synthetase, and homoserine dehydrogenase by aspartic acid family amino acids in Streptomyces clavuligerus. Antimicrobial Agents and Chemotherapy 21:74–84
    [Google Scholar]
  13. Sano K., Shiio I. 1970; Microbial production of L-lysine. III. Production by mutants resistant to S-(2-aminoethyl)-L-cysteine. Journal of General and Applied Microbiology 16:373–391
    [Google Scholar]
  14. Shabtai Y., Rosenberg E., Kindler S.H. 1975; Aspartokinaseisoenzymes of the fruiting Myxobacterium Myxococcus xanthus. Biochimica et biophysica acta 403:345–354
    [Google Scholar]
  15. Shiio I., Miyajima R. 1969; Concerted inhibition and its reversal by end product of aspartate kinase in Brevibacterium flavum. Journal of Biochemistry 65:849–859
    [Google Scholar]
  16. Stadtman E.R., Cohen G.N., Le-Bras G., De Robichon-Szulmajster H. 1961; Feedback inhibition and repression of aspartokinase activity in Escherichia coli and Saccharomyces cerevisiae. Journal of Biological Chemistry 236:2033–2038
    [Google Scholar]
  17. Umbarger H.E. 1978; Amino acid biosynthesis and its regulation. Annual Review of Biochemistry 41:533–606
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-7-2063
Loading
/content/journal/micro/10.1099/00221287-129-7-2063
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error