1887

Abstract

grown under different specific nutrient depletions in batch culture showed varying degrees of sensitivity to engulfment and killing by human polymorphonuclear leukocytes () and to killing by human serum. Resistance to killing by the combined action of and serum in whole blood was found to increase in the following order of depletions: glucose < iron < sulphate < phosphate or ammonium < magnesium. There was also an increase in resistance to killing by whole blood with decrease in temperature, except that carbon-depleted cells remained very sensitive irrespective of temperature. Cells in the exponential phase of growth also showed a consistent increase in resistance as the temperature was decreased. Similar, but smaller effects were observed with oxygen-depleted cells. The increase in killing by whole blood as the phagocytic temperature was raised correlated with an increase in the number of bacteria ingested per . A pattern of serum sensitivity was observed with cells grown under different nutrient depletions similar to that for whole blood. But in all cases whole blood was 6 to 10 times more effective than serum alone in killing the cells at 37 °C.

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1983-07-01
2021-10-27
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References

  1. Anwar H., Brown M.R.W., Cozens R.M., Lambert P.A. 1983; Isolation and characterization of the outer and cytoplasmic membranes of Pseudomonas cepacia. Journal of General Microbiology 129:499–507
    [Google Scholar]
  2. Ballard R.W., Palleroni N.J., Doudoroff M., Stanier R.Y., Mandel M. 1970; Taxonomy of the aerobic Pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. Journal of General Microbiology 60:199–214
    [Google Scholar]
  3. Beveridge T.J. 1980; Bacterial structure and its implications in the mechanisms of infection: a short review. Canadian Journal of Microbiology 26:643–653
    [Google Scholar]
  4. Brown M.R.W. 1975; The role of the cell envelope in resistance. In Resistance of Pseudomonas aeruginosa pp. 71–107 Edited by Brown M. R. W. London: John Wiley;
    [Google Scholar]
  5. Brown M.R.W. 1977; Nutrient depletion and antibiotic susceptibility. Journal of Antimicrobial Chemotherapy 3:198–201
    [Google Scholar]
  6. Bullen J.J. 1981; The significance of iron in infection. Reviews of Infectious Diseases 3:1127–1138
    [Google Scholar]
  7. Costerton J.W., Brown M.R.W., Sturgess J.M. 1979; The cell envelope: its role in infection. In Pseudomonas aeruginosa: Clinical Manifestation of Infection and Current Therapy pp. 41–62 Edited by Doggett R. G. New York: Academic Press;
    [Google Scholar]
  8. Day D.F., Marceau-Day M.L. 1982; Lipopolysaccharide variability in Pseudomonas aeruginosa. Current Microbiology 7:93–98
    [Google Scholar]
  9. Finch J.E., Brown M.R.W. 1978; Effect of growth environment on Pseudomonas aeruginosa killing by rabbit polymorphonuclear leukocytes and cationic proteins. Infection and Immunity 20:340–346
    [Google Scholar]
  10. Garvey G.J., Neu H.C. 1975; Infective endocarditis. Postgraduate Medicine 58:107–113
    [Google Scholar]
  11. Goldman R.C., Leive L. 1980; Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli0lll and Salmonella typhimurium LT2. European Journal of Biochemistry 107:145–153
    [Google Scholar]
  12. Jones R.J., Roe E.A., Gupta J.L. 1979; Controlled trials of a polyvalent Pseudomonas vaccine in burns. Lancet ii:977–983
    [Google Scholar]
  13. Mackowiak P.A., Marling-Caron M., Cohen R.L. 1982; Effects of temperature on antimicrobial susceptibility of bacteria. Journal of Infectious Diseases 145:550–553
    [Google Scholar]
  14. Mandell G.L. 1975; Effect of temperature on phagocytosis by human polymorphonuclear neutrophils. Infection and Immunity 12:221–223
    [Google Scholar]
  15. Miles A.A., Misra S.S. 1938; The estimation of the bactericidal power of blood. Journal of Hygiene 38:732–748
    [Google Scholar]
  16. Palva E.T., Makela P.H. 1980; Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. European Journal of Biochemistry 107:137–143
    [Google Scholar]
  17. Phillips I., Eykyn S. 1971; Pseudomonas cepacia (multivorans) septicemia in an intensive care unit. Lancet i:375–377
    [Google Scholar]
  18. Roberts N.J. 1979; Temperature and host defence. Microbiological Reviews 43:241–259
    [Google Scholar]
  19. Roberts N.J., Steigbigel R.T. 1977; Hyperthermia and human leukocyte functions: effects on response of lymphocytes to mitogen and antigen and bactericidal capacity of monocytes and neutrophils. Infection and Immunity 18:673–679
    [Google Scholar]
  20. Smith H. 1977; Microbial surfaces in relation to pathogenicity. Bacteriological Reviews 41:475–500
    [Google Scholar]
  21. Speller D.G., Stephens M.E., Viant A.C. 1971; Hospital infection by Pseudomonas cepacia. Lancet i:798–799
    [Google Scholar]
  22. Stendahl L., Norman B., Edebo L. 1979; Influence of O and K antigens on the surface properties of E. coli in relation to phagocytosis. Actapathologica scandinavica, Section B 87:85–91
    [Google Scholar]
  23. Van Oss C.J. 1978; Phagocytosis as a surface phenomenon. Annual Review of Microbiology 32:19–39
    [Google Scholar]
  24. Van Oss C.J., Gillman C.F., Neumann A.W. 1975 Phagocytic Engulfment and Cell Adhesiveness as Cellular Surface Phenomena. New York: Marcel Dekker;
    [Google Scholar]
  25. Weinberg E.D. 1978; Iron and infection. Microbiological Reviews 42:45–66
    [Google Scholar]
  26. Weinstein R.A., Emori T.G., Anderson R.L., Stamm W.E. 1976; Pressure transducers as a source of bacteremia after open heart surgery.Report of outbreak and guidelines for prevention. Chest 69:338–344
    [Google Scholar]
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