Amino Acid Requirements of Strains of Chlamydia trachomatis and C. psittaci Growing in McCoy Cells: Relationship with Clinical Syndrome and Host Origin
† Present address: Department of Microbiology and Immunology, UCLA School of Medicine, Center for Health Sciences, Los Angeles, California 90024, U.S.A.
The effects of omission of individual amino acids from growth medium on the multiplication of a range of Chlamydia trachomatis and C. psittaci strains in cycloheximide-treated McCoy cells have been assessed. Differences in requirements were revealed which for C. trachomatis strains correlated with clinical syndrome and for C. psittaci with host origin. All 11 strains of C. trachomatis examined showed a requirement for addition of histidine to the medium; this was not shown by any of four C. psittaci strains. Among the strains of C. trachomatis, three from cases of trachoma, representing serotypes A, B and C, showed a distinctive requirement for the addition of tryptophan to the medium, whilst six strains of oculogenital origin, representing serotypes D-I, exhibited no requirement for tryptophan or methionine; a lymphogranuloma venereum and a ‘fast variant’ strain both showed a requirement for methionine. Of the four C. psittaci strains from different hosts, three showed distinct patterns of amino acid requirements. All chlamydiae squired the addition of valine to medium and the majority required leucine, phenylalanine and also glutamine.
AllanI., PearceJ.H.1983; Differential amino acid utilization by Chlamydia psittaci (strain guinea pig inclusion conjunctivitis) and its regulatory effect on chlamydial growth. Journal of General Microbiology 129:1991–2000
CollierL.H., SmithA.1967; Dissemination and immunogenicity of live TRIC agent in baboons after parenteral injection. American Journal of Ophthalmology 63:1589–1602
DarougarS., MonnickendamM.A., El-SheikhH., TreharneJ.D., WoodlandR.M., JonesB.R.1977; Animal models for the study of chlamydial infections of the eye and genital tract. In Nongonococcal Urethritis and Related Infections pp. 186–198 Edited by HolmesK.K., HobsonD. Washington: American Society for Microbiology;
FrancisT, MagillT.P. 1938; An unidentified virus producing acute meningitis and pneumonitis in experimental animals. Journal of Experimental Medicine 68:147–160
GriffithsM.S, AinsworthS, PearceJ.H. 1976; Infectivity titration of guinea-pig inclusion conjunctivitis agent in irradiated McCoy cells. Journal of General Microbiology 95:249–256
HowieJ.ChairmanChairmanCode of Practice for Prevention of Infection in Clinical Laboratories and Postmortem Rooms London: Her Majesty’s Stationery Office;
KuoC.C., WangS.P., GraystonJ.T. 1977; Growth of trachoma organisms in HeLa-229 cell cultures. In Nongonoccal Urethritis and Related Infections pp 328–336 Edited by HolmesK.K, HobsonD. Washington: American Society for Microbiology;
MurrayE.S. 1964; Guinea pig inclusion conjunctivitis virus. I. Isolation and identification as a member of the psittacosis-lymphogranuloma- trachoma group. Journal of Infectious Diseases 114:1–12
QuayS.C, OxenderD.L. 1980; Regulation of membrane transport. In Biological Regulation and Development2 pp 413–436 Edited by GoldbergerR.F. New York: Plenum Press;
TischlerM.E, DesautelsM, GoldbergA.L. 1982; Does leucine, leucyl-tRNA, or some metabolite of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle?. Journal of Biological Chemistry 257:1613–1621
TreharneJ.D, DinesR.J, DarougarS. 1977; Serological responses to chlamydial ocular and genital infections in the United Kingdom and Middle East. In Nongonococcal Urethritis and Related Infections pp 249–258 Edited by HolmesK.K, HobsonD. Washington: American Society for Microbiology;
WangS.P, GraystonJ.T. 1971a; Classification of TRIC and related strains with micro-immunofluorescence. In Trachoma and Related Disorders pp 305–321 Edited by NicholsR.L. Amsterdam: Excerpta Medica;
WangS.P, GraystonJ.T. 1971b; Studies on the identity of the ‘fast’ egg-killing chlamydia strains. In Trachoma and Related Disorders pp 322–336 Edited by NicholsR.L. Amsterdam: Excerpta Medica;
Amino Acid Requirements of Strains of Chlamydia trachomatis and C. psittaci Growing in McCoy Cells: Relationship with Clinical Syndrome and Host Origin