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Room temperature CO-difference spectra of whole cells ofCrithidia fasciculata show two CO-reactinghaemoproteins. The reaction of cytochrome a/a 3 with CO is complete within 1 min of bubbling with CO; that of cytochrome b takes longer than 40 min. A non-photodissociable O2-containing compound of cytochrome a/a 3 was formed in whole cell suspensions at −112 °C after photolysis of CO in the presence of 200 μm-O2. No O2-cytochrome b compound was observed under these conditions. Photochemical action spectra for the relief of CO-inhibited respiration, obtained at different O2 tensions, indicate cytochrome a/a 3 to be the major haemoprotein terminal oxidase; no evidence for a b-type cytochrome oxidase has been found.
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