SUMMARY: Room temperature CO-difference spectra of whole cells of show two CO-reacting haemoproteins. The reaction of cytochrome with CO is complete within 1 min of bubbling with CO; that of cytochrome takes longer than 40 min. A non-photodissociable O-containing compound of cytochrome was formed in whole cell suspensions at — 112 °C after photolysis of CO in the presence of 200 μm-O. No O-cytochrome compound was observed under these conditions. Photochemical action spectra for the relief of CO-inhibited respiration, obtained at different O tensions, indicate cytochrome to be the major haemoprotein terminal oxidase; no evidence for a -type cytochrome oxidase has been found.


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