1887

Abstract

The role of NH in the regulation of sporulation in has been studied by analysing the effects of NH and methylammonium ions on sporulation in a wild-type strain; a strain homozygous for the mutation, (derepressed sporulation on a number of carbon sources, reduced sensitivity to NH -inhibition of sporulation); and a strain homozygous for the mutation, (the structural gene for the anabolic NADP-dependent glutamate dehydrogenase). The addition of ammonium or methylammonium ions to sporulation medium resulted in incomplete ascus formation. The mutation resulted in a loss of sensitivity to NH -inhibition of initiation of sporulation. At higher concentrations of NH the strain homozygous for the mutation was even less sensitive than the sporulation-derepressed strain. However, in the formation of complete asci all three strains behaved very similarly over the whole range of ammonium sulphate concentration. These studies indicate there are at least two separate stages affected by NH ; one early, possibly initiation, the other later, concerned with the organization and delimitation of mature spores. From the reduced sensitivity to NH -inhibition of initiation of sporulation conferred by the mutation, and the fact that similar results were obtained using methylammonium ion, it is concluded that some metabolite of NH is responsible rather than NH itself. In addition, the data provide some insight into the nature of the spdl mutation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-6-1883
1983-06-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/6/mic-129-6-1883.html?itemId=/content/journal/micro/10.1099/00221287-129-6-1883&mimeType=html&fmt=ahah

References

  1. Croes A. F., Steijns J. M., De Vries G., Van Der Putte T. M. 1978; Inhibition of meiosis in Saccharomyces cerevisiae by ammonium ions: interference of ammonia with protein metabolism. Planta 141:205–209
    [Google Scholar]
  2. Dawes I. W. 1975; Study of cell development using derepressed mutations. Nature, London 255:707–708
    [Google Scholar]
  3. Dawes I. W., Hardie I. H. 1974; Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Molecular and General Genetics 131:281–289
    [Google Scholar]
  4. Delavier-Klutchko C., Durieu-Trautmann O. 1978; Effect of ammonia on glycogen metabolism during sporulation of Saccharomyces cerevisiae. Journal of General Microbiology 108:169–171
    [Google Scholar]
  5. Delavier-Klutchko C., Durieu-Trautmann O., Allemand P., Tavlitzki J. 1980; Assimilation of ammonia during sporogenesis of Saccharomyces cerevisiae: effect of ammonia and glutamine. Journal of General Microbiology 116:143–148
    [Google Scholar]
  6. Dubois E., Vissers S., Grenson M., Wiame J-M. 1977; Glutamine and ammonia in nitrogen catabolite repression of Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 75:233–239
    [Google Scholar]
  7. Durieu-Trautmann O., Delavier-Klutchko C. 1977; Effect of ammonia and glutamine on macro-molecular synthesis and breakdown during sporulation of Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 79:438–442
    [Google Scholar]
  8. Fonzi W. A., Shanley M., Opheim D. J. 1979; Relationship of glycolytic intermediates, glycolytic enzymes, and ammonia to glycogen metabolism during sporulation in the yeast Saccharomyces cerevisiae. Journal of Bacteriology 137:285–294
    [Google Scholar]
  9. Gosling J. P., Duggan P. F. 1971; Activities of tricarboxylic acid cycle enzymes, glyoxylate cycle enzymes and fructose diphosphatase in baker’s yeast during adaptation to acetate oxidation. Journal of Bacteriology 106:908–914
    [Google Scholar]
  10. Grenson M., Dubois E., Piotrowska M. 1974; Ammonia assimilation in Saccharomyces cerevisiae as mediated by the two glutamate dehydrogenases. Evidence for the gdhA locus being a structural gene for the NADP-dependent glutamate dehydrogenase. Molecular and General Genetics 128:73–85
    [Google Scholar]
  11. Illingworth R. F. 1973; Lipid composition of Saccharomyces cerevisiae DCL740 in relation to morphogenesis of ascospores. Ph.D. thesis University of Bath. Bath; U.K.:
    [Google Scholar]
  12. Mazón M. J. 1978; Effect of glucose starvation on the nicotinamide adenine dinucleotide phosphate- dependent glutamate dehydrogenase of yeast. Journal of Bacteriology 133:780–785
    [Google Scholar]
  13. Mazón M. J., Hemmings B. A. 1979; Regulation of Saccharomyces cerevisiae nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase by proteolysis during carbon starvation. Journal of Bacteriology 139:686–689
    [Google Scholar]
  14. Miller J. J. 1963; The metabolism of yeast sporulation. V. Stimulation and inhibition of sporulation and growth by nitrogen compounds. Canadian Journal of Microbiology 9:259–277
    [Google Scholar]
  15. Newlon M. C. 1979; NADP-specific glutamate dehydrogenase is not involved in repression of yeast sporulation by ammonia. Molecular and General Genetics 176:297–300
    [Google Scholar]
  16. Piñon R. 1977; Effects of ammonium ions on sporulation of Saccharomyces cerevisiae. Experimental Cell Research 105:367–378
    [Google Scholar]
  17. Vezinhet F., Kinnaird J. H., Dawes I. W. 1979; The physiology of mutants derepressed for sporulation in Saccharomyces cerevisiae. Journal of General Microbiology 115:391–402
    [Google Scholar]
/content/journal/micro/10.1099/00221287-129-6-1883
Loading
/content/journal/micro/10.1099/00221287-129-6-1883
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error