1887

Abstract

Extracellular endocellulase production by closely paralleled mycelial growth in cultures containing microcrystalline cellulose. The enzyme was induced by various celluloses and cellobiose. In the presence of a cellulose inducer glucose and cellobiose repressed production of the enzyme. Endocellulase activity in culture filtrates was inversely related to cellulose concentration in the culture. In high concentrations of cellulose the activity of free enzyme was low. Evidence was obtained for the existence of two forms of endocellulase activity. One form adsorbed strongly to cellulose and was predominant in cultures low in cellulose. In cultures with a high cellulose content a non-adsorbable form of the enzyme was more abundant. The regulation of the appearance of free enzyme in relation to cellulose content and enzyme adsorption is discussed.

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1983-06-01
2021-07-30
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