1887

Abstract

Conditions for the regeneration of cells from protoplasts of a producer of the peptide antibiotic actinomycin, are described. Regeneration of fusion products was most efficient at 27–30 °C on regeneration R2 medium ( Okanishi ., 1974 ) containing 0·25 -sucrose. The addition of phosphate (150–300 mg 1) to the medium and incubation at 23 °C proved to be optimal for the regeneration of individual strains. Highest recombination frequencies after protoplast fusion were obtained by fusing protoplasts in the presence of 45% (w/v) polyethylene glycol 6000. With strains that produce no, or little antibiotic, protoplasts must be present in excess in fusion mixtures in order to overcome inhibition of regeneration by the antibiotic-producing partner.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-6-1725
1983-06-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/6/mic-129-6-1725.html?itemId=/content/journal/micro/10.1099/00221287-129-6-1725&mimeType=html&fmt=ahah

References

  1. Baltz R. H. 1978; Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. Journal of General Microbiology 107:93–102
    [Google Scholar]
  2. Baltz R. H. 1980; Genetic recombination by protoplast fusion in Streptomyces. Developments in Industrial Microbiology 21:43–54
    [Google Scholar]
  3. Baltz R. H., Matsushima P. 1981; Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration. Journal of General Microbiology 127:137–146
    [Google Scholar]
  4. DeliĆ V., Hopwood D. A., Friend E. J. 1970; Mutagenesis by N-methyl-N′-nitro-N-nitroso-guanidine (NTG) in Streptomyces coelicolor. Mutation Research 9:167–182
    [Google Scholar]
  5. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriological Reviews 31:373–403
    [Google Scholar]
  6. Hopwood D. A., Wright H. M. 1978; Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor. Molecular and General Genetics 162:307–317
    [Google Scholar]
  7. Hopwood D. A., Wright H. M., Bibb M. J., Cohen S. N. 1977; Genetic recombination through protoplast fusion in Streptomyces. Nature; London: 268171–174
    [Google Scholar]
  8. Hori M., Hemmi H., Suzukake K., Hayashi H., Uehava Y., Takeuchi T., Umezawa H. 1978; Biosynthesis of leupeptin. Journal of Antibiotics 31:96–98
    [Google Scholar]
  9. Katz E. 1967; Actinomycin. In Antibiotics II. Biosynthesis pp. 276–341 Gottlieb D., Shaw P. D. Edited by New York:: Springer Verlag.;
    [Google Scholar]
  10. Katz E., Weissbach H. 1962; Biosynthesis of the actinomycinchromophore.Enzymatic conversion of 4-methyl-3-hydroxy-anthranilic acid to actinocin. Journal of Biological Chemistry 237:882–886
    [Google Scholar]
  11. Katz E., Weissbach H. 1963; Incorporation of C14-labeled amino acids into actinomycin and protein by Streptomyces antibioticus. Journal of Biological Chemistry 238:666–675
    [Google Scholar]
  12. Keller U., Kleinkauf H. 1977; Studies of the biosynthesis of actinomycin in protoplasts from Streptomyces antibioticus. Archives of Biochemistry and Biophysics 184:111–124
    [Google Scholar]
  13. Keller U., Ebert M., Kleinkauf H. 1982; Genetic studies on the biosynthesis of actinomycin. In Peptide Antibiotics-Biosynthesis and Functions pp. 101–108 Kleinkauf H., Dohren H. v. Edited by Berlin & New York:: Walter de Gruyter.;
    [Google Scholar]
  14. Ochi K. 1982; Control of the actinomycin biosynthetic pathway in and actinomycin resistance of Streptomyces spp. Journal of Bacteriology 150:598–603
    [Google Scholar]
  15. Okanishi M., Suzuki K., Umezawa H. 1974; Formation and reversion of streptomyceteprotoplasts : cultural condition and morphological study. Journal of General Microbiology 80:389–400
    [Google Scholar]
  16. Yoshida T., Weissbach H., Katz E. 1966; Inhibitory effects of actinomycin upon the producing organism. Archives of Biochemistry and Biophysics 114:252–255
    [Google Scholar]
  17. Zocher R., Keller U., Kleinkauf H. 1982; Enniatinsynthetase, a novel type of multifunctional enzyme catalyzing depsipeptide synthesis in Fusarium oxysporum. Biochemistry 21:43–48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-6-1725
Loading
/content/journal/micro/10.1099/00221287-129-6-1725
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error