%0 Journal Article %A Lu, Wei-Ping %A Kelly, D. P. %T Partial Purification and Resolution of a Thiosulphate-oxidizing System from Thiobacillus A2 %D 1983 %J Microbiology, %V 129 %N 6 %P 1673-1681 %@ 1465-2080 %R https://doi.org/10.1099/00221287-129-6-1673 %I Microbiology Society, %X A crude thiosulphate-oxidizing extract of Thiobacillus A2 has been resolved by ammonium sulphate fractionation and DEAE-Sepharose CL-6B chromatography into fractions containing several components necessary for thiosulphate oxidation and reduction of cytochrome c. These components include two distinct c-type cytochromes, sulphite: cytochrome c oxidoreductase, rhodanese and enzymes probably responsible for thiosulphate cleavage and sulphane-sulphur oxidation. The complete cytochrome c-dependent thiosulphate-oxidizing system could be reconstituted by mixing selected fractions from the column chromatography. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-6-1673