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Abstract
A crude thiosulphate-oxidizing extract of Thiobacillus A2 has been resolved by ammonium sulphate fractionation and DEAE-Sepharose CL-6B chromatography into fractions containing several components necessary for thiosulphate oxidation and reduction of cytochrome c. These components include two distinct c-type cytochromes, sulphite: cytochrome c oxidoreductase, rhodanese and enzymes probably responsible for thiosulphate cleavage and sulphane-sulphur oxidation. The complete cytochrome c-dependent thiosulphate-oxidizing system could be reconstituted by mixing selected fractions from the column chromatography.
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© Society for General Microbiology, 1983