%0 Journal Article %A Ingledew, W. John %T The Electron Transport Chain of Escherichia coli Grown Anaerobically with Fumarate as Terminal Electron Acceptor: an Electron Paramagnetic Resonance Study %D 1983 %J Microbiology, %V 129 %N 6 %P 1651-1659 %@ 1465-2080 %R https://doi.org/10.1099/00221287-129-6-1651 %I Microbiology Society, %X The electron transport chain of Escherichia coli grown anaerobically on glycerol with fumarate as terminal electron acceptor, has been studied using electron paramagnetic resonance (EPR) spectroscopy. The analysis did not include cytochromes, but was confined to the lower potential (dehydrogenase) section of the electron transport chain. Several ferredoxin-type centres were detected and partially characterized, and the possible presence of iron-sulphur centres paramagnetic in their oxidized form (‘HiPIP-type’) was also noted. An EPR-detectable signal that may have been due to the presence of molybdenum in the electron transport chain is described and assessed. Most of the centres detected were reducible by substrate in the absence of oxidant and some were found to be wholly or partly reduced during steady state oxidation of substrates in the presence of oxygen. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-6-1651