RT Journal Article SR Electronic(1) A1 Wessels, J. G. H. A1 Sietsma, J. H. A1 Sonnenberg, A. S. M.YR 1983 T1 Wall Synthesis and Assembly During Hyphal Morphogenesis in Schizophyllum commune JF Microbiology, VO 129 IS 6 SP 1607 OP 1616 DO https://doi.org/10.1099/00221287-129-6-1607 PB Microbiology Society, SN 1465-2080, AB Pulse-chase experiments with [14Cglucose suggested that in growing hyphae of Schizophyllum commune, a water-soluble β-glucan is a precursor for the alkali-insoluble chitin-β-glucan complex in the wall. With microautoradiography it was found that this complex was not present at the very tip of the growing hyphae where chitin and β-glucan were inserted into the wall as individual polymers. The two polymers were then gradually turned into an insoluble complex, probably by covalent cross-linking. This was also observed in the entire hyphal apex after arrestment of growth. Evidence is presented which indicates that complex formation affects the mechanical properties of the wall, and a model is given in which the formation of a complex between chitin and β-glucan accounts for the change from plasticity towards rigidity of the wall during hyphal morphogenesis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-6-1607