α-Factor Enhancement of Hybrid Formation by Protoplast Fusion in the Yeast Free

Abstract

Two a strains of carrying complementary genetic markers, were arrested for 3 h with α-factor. These were then protoplasted, prior to fusion using polyethylene glycol. The number of viable fusion products was enhanced by a factor of 20 as compared with unarrested controls.

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/content/journal/micro/10.1099/00221287-129-5-1589
1983-05-01
2024-03-29
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