1887

Abstract

A P NMR study of the fungal pathogen was carried out. Yeast-form cells at different phases of growth, as well as germ tubes and hyphae were examined. In all cases, the NMR spectra showed well separated resonance peaks arising from phosphorus-containing metabolites, the most prominent being attributable to inorganic phosphate (P) polyphosphates, sugar phosphates and mononucleotides, NAD, ADP and ATP. Relevant signals were also detected in the phosphodiester region. The intensity of most signals, as measured relative to that of P, was clearly modulated both at the different phases of growth and during yeast-to-mycelium conversion, suggesting significant changes in the intracellular concentration of the corresponding metabolites. In particular, the intensity of the polyphosphate signal was high in exponentially growing, yeast-form cells, then progressively declined in the stationary phase, was very low in germ tubes and, finally, undetectable in hyphae.

NMR spectral analysis of the P region showed that from early-stationary phase, P was present in two different cellular compartments, probably corresponding to the cytoplasm and the vacuole. From the chemical shift of P, the pH values of these two compartments could be evaluated. The cytoplasmic pH was generally slightly lower than neutrality (6·7–6·8), whereas the vacuolar pH was always markedly more acidic.

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1983-05-01
2024-04-18
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References

  1. Burt C. T., Cohen S. M., Bárány M. 1979; Analysis of intact tissues with 31P NMR. Annual Review of Biophysics and Bioengineering 8:1–25
    [Google Scholar]
  2. Cassone A., Simonetti N., Strippoli V. 1973; Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans. Journal of General Microbiology 77:417–426
    [Google Scholar]
  3. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans. Journal of General Microbiology 51:367–376
    [Google Scholar]
  4. Chattaway F. W., Bishop R., Holmes M. R., Odds F. C., Barlow A. J. E. 1973; Enzyme activities associated with carbohydrate synthesis and breakdown in yeast and mycelial forms of Candida albicans. Journal of General Microbiology 75:97–109
    [Google Scholar]
  5. Costello A. J. R., Glonek T., Slodki M. E., Seymour F. R. 1975; Phosphorus 31 nuclear magnetic resonance spectroscopy of extracellular yeast O-phosphonohexoglucans. Carbohydrate Research 42:23–37
    [Google Scholar]
  6. Gadian D. G. 1982 Nuclear Magnetic Resonance and its Applications to Living Systems. Oxford:: Clarendon Press.;
    [Google Scholar]
  7. Gadian D. G., Radda G. K. 1981; NMR studies of tissue metabolism. Annual Review of Biochemistry 50:69–83
    [Google Scholar]
  8. Harold F. M. 1966; Inorganic polyphosphate in biology: structure, metabolism and function. Bacteriological Reviews 30:772–794
    [Google Scholar]
  9. Hollis D. P. 1980; Phosphorus NMR of cells, tissues and organelles. In Biological Magnetic Resonance 2 pp. 1–44 Berliner L. J., Reuben J. Edited by New York & London:: Plenum Press.;
    [Google Scholar]
  10. Indge K. J. 1968; Polyphosphates of the yeast cell vacuole. Journal of General Microbiology 51:447–455
    [Google Scholar]
  11. Land G. A., Mcdonald W. C., Stjernholn R. L., Friedman L. 1975; Factors affecting filamentation in Candida albicans. Changes in respiratory activity of Candida albicans during filamentation. Infection and Immunity 12:119–127
    [Google Scholar]
  12. Lodder J. 1970 The Yeasts: a Taxonomic Study pp. 914–978 Amsterdam:: North Holland.;
    [Google Scholar]
  13. Marriott M. S. 1975; Isolation and chemical characterization of plasma membrane from the yeast and the mycelial forms of Candida albicans. Journal of General Microbiology 86:115–132
    [Google Scholar]
  14. Matile P. H., Moor H., Robinow C. F. 1969 Yeast Cytology.In The Yeasts I pp. 219–302 Rose A. H., Harrison J. S. Edited by London & New York:: Academic Press.;
    [Google Scholar]
  15. Mattia E., Cassone A. 1979; Inducibility of germ-tube formation in Candida albicans at different phases of yeast growth. Journal of General Microbiology 113:439–442
    [Google Scholar]
  16. Moon R. B., Richards J. H. 1973; Determination of intracellular pH by 31P magnetic resonance. Journal of Biological Chemistry 248:7276–7278
    [Google Scholar]
  17. Navon G., Shulman R. G., Yamane T., Rosseccleshall T., Lam K. B., Baronowsky J. J., Marmur J. 1979; Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae. Biochemistry 18:4487–4499
    [Google Scholar]
  18. Odds F. C. 1979 Candida and Candidosis. Leicester:: University Press.;
    [Google Scholar]
  19. Radda G. K., Seeley P. J. 1979; Recent studies on cellular metabolism by nuclear magnetic resonance. Annual Review of Physiology 41:749–769
    [Google Scholar]
  20. Roberts J. K. M., Ray P. M., Wade-Jardetzky N., Jardetzky O. 1980; Estimation of cytoplasmic and vacuolar pH in higher plant cells by 31P NMR. Nature; London: 283870–872
    [Google Scholar]
  21. Roberts J. K. M., Wade-Jardetzky N., Jardetzky O. 1981; Intracellular pH measurements by 31P nuclear magnetic resonance. Influence of factors other than pH on 31P chemical shifts. Bio-chemistry 20:5389–5394
    [Google Scholar]
  22. Salhany J. M., Yamane T., Shulman R. G., Ogawa S. 1975; High resolution 31P nuclear magnetic resonance studies of intact yeast cells. Proceedings of the National Academy of Sciences of the United States of America 72:4966–4970
    [Google Scholar]
  23. Shulman R. G., Brown T. R., Ugurbil K., Ogawa S., Cohen S. M., Denhollander J. A. 1979; Cellular applications of 31P and 13C nuclear magnetic resonance. Science 205:160–166
    [Google Scholar]
  24. Simonetti N., Strippoli V., Cassone A. 1974; Yeast-mycelial conversion induced by N-acetyl-d-glucosamine in Candida albicans. Nature; London: 250344–346
    [Google Scholar]
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