1887

Abstract

With radioisotopes, it was shown that suspensions of oxidized glycerol, 6-phosphogluconate, glucose, glucose 6-phosphate, and, at a low rate, gluconate, to CO. The incubation period in these experiments was usually 20 h, but after 140 h up to five times more glucose and gluconate had been converted to CO. Studies with differentially labelled glucose indicated that glycolysis and the hexose monophosphate pathway were used for glucose dissimilation.

Key enzymes of glycolysis, the hexose monophosphate pathway and glycerol catabolism were detected in cell-free extracts from purified but phosphoketolase, Entner-Doudoroff pathway activity and gluconate kinase were absent. All these enzymes were present also in host-tissue, but biochemical evidence is presented which indicates that all enzymes detected in extracts from were authentic bacterial enzymes. Additionally, they could all be detected in extracts of prepared by treatment with NaOH in which host enzymes adsorbed to are inactivated.

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1983-05-01
2021-10-24
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