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Abstract
Millipore filters placed underneath agar block cultures of Phytophthora parasitica were able to adsorb hormones α1 and α2 produced by A1 and A2 mating types of the fungus, respectively. Both hormones were released from moistened Millipore filters by extraction with ether, which on evaporation resulted in aqueous hormone extracts. Isolated hormones α1 and α2 stimulated oospore formation by A2 and A1 mating types of P. parasitica, respectively, when they were adsorbed on small pieces of Millipore filter, but not when they were added directly to agar cultures. Hormones α1 and α2 were removed from aqueous solutions by both cation and anion exchange resins, and could be eluted from the resins by ether. Both hormones α1 and α2 were able to pass through membranes with a molecular weight cut-off of 1000 and 500, but not 100 and 50.
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