1887

Abstract

Adherence of C-labelled strains of to uncoated and saliva-coated silica and hydroxyapatite beads had both loose and firm components, probably reflecting different subpopulations of bacteria within a single culture. Adherence was characterized by the proportion of bacteria available for each type of adherence and a constant ( ) for each combination of bacterial strain and bead surface. Loose adherence, which was greater with silica than with hydroxyapatite beads, always involved many more bacteria than firm adherence. Firm adherence was greater with WVU627 than TF11. The association rate constants ( ) for loose and firm adherence were similar, indicating simultaneous processes, but the dissociation rate constant ( ) was lower for loose adherence than for firm adherence. Removal of loosely adhering bacteria by washing may only reflect their distance from the bead surface. Silica beads were convenient for studying bacterial adherence and formed an acceptable coating of salivary glycoprotein.

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/content/journal/micro/10.1099/00221287-129-5-1387
1983-05-01
2024-04-24
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References

  1. Clark W. B., Bammann L. L., Gibbons R. J. 1978; Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infection and Immunity 19:846–853
    [Google Scholar]
  2. Embery G., Hogg S. D. 1981; Some considerations on the interaction between mucous glycoproteins and oral streptococci. In Tooth Surface Interactions and Preventative Dentistry pp. 83–94 Edited by Rolla G., Sonju T., Embery G. London: IRL Press;
    [Google Scholar]
  3. Feltham R. K. A., Power A. K., Pell P. A., Sneath P. H. A. 1978; A simple method for storage of bacteria at −76°C. Journal of Applied Bacteriology 44:313–316
    [Google Scholar]
  4. Fillery E. E., Bowden G. H., Hardie J. M. 1978; A comparison of strains of bacteria designatedActinomyces viscosus andActinomyces naeslundii. Caries Research 12:299–312
    [Google Scholar]
  5. Gibbons R. J., Van Houte . 1975; Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29:19–44
    [Google Scholar]
  6. Hogg S. D., Embery G. 1979; The isolation and partial characterisation of a sulphated glycoprotein from human whole saliva which aggregates strains ofStreptococcus sanguis but notStreptococcus mutans. Archives of Oral Biology 24:791–797
    [Google Scholar]
  7. Jordan H. V., Keyes P. H. 1964; Aerobic Grampositive filamentous bacteria as etiologic agents of experimental peridontal disease in hamsters. Archives of Oral Biology 9:401–414
    [Google Scholar]
  8. Rolla G. 1977; Formation of dental integuments - some basic chemical considerations. Swedish Dental Journal 1:241–251
    [Google Scholar]
  9. Russell C., Jones C. 1980; Interactions in adhesion of bacteria to a toothin vitro. In Dental Plaque and Surface Interactions in the Oral Cavity pp. 145–154 Edited by Leach S. A. London: IRL Press;
    [Google Scholar]
  10. Shetlar M. R., Masters Y. F. 1957; Use of thymol-sulphuric acid reaction for determination of carbohydrates in biological material. Analytical Chemistry 29:402–405
    [Google Scholar]
  11. Staat R. H., Langley S. D., Doyle R. J. 1980; Streptococcus mutans adherence: presumptive evidence for protein mediated attachment followed by glucan dependent cellular accumulation. Infection and Immunity 27:675–681
    [Google Scholar]
  12. Stinson M. W., Jinks D. C., Merrick J. M. 1981; Adherence ofStreptococcus mutans andStreptococcus sanguis to salivary components bound to glass. Infection and Immunity 32:583–591
    [Google Scholar]
  13. Sumney D. L., Jordan H. V. 1974; Characterisation of bacteria isolated from human root surface carious lesions. Journal of Dental Research 53:343–351
    [Google Scholar]
  14. Weerkamp A. H., Mcbride B. C. 1980; Adherence ofStreptococcus salivarius HB and HB-7 to oral surface and saliva coated hydroxyapatite. Infection and Immunity 30:150–158
    [Google Scholar]
  15. Wheeler T. T., Clark W. B., Birdsell D. C. 1979; Adherence ofActinomyces viscosus T14V and T14AV to hydroxyapatite surfacesin vitro and human teethin vivo. Infection and Immunity 25:1066–1074
    [Google Scholar]
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