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Several differences and similarities between the macrocyst and fruiting body pathways of development are described. First, as has been long assumed, starvation initiates the development of amoebae for both pathways. Second, amoebae make the decision to produce macrocysts or fruiting bodies before a critical time point approximately 12 h after the onset of starvation. After this time, amoebae are committed to fruiting body development. Substances that can push amoebae into one or the other pathway often do so by altering the timing of development. Finally, cell division may be required in order for amoebae to become competent to produce macrocysts; there is no such requirement for fruiting body development.
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