Summary: Three cutaneous propionibacteria, and were grown in chemostats using semi-synthetic medium at various pH values. Growth occurred between pH 4.5 and 7.5 for and pH 5.0 and 8.0 for and The highest μ was at pH 6.0 for the three species. Maximum biomass production was obtained at pH 6.0 for and and at pH 7.5 for Extracellular enzyme production occurred over the entire pH growth range when denaturation of the enzymes was taken into account. However, detectable activity of the enzymes was found in a narrower range of pH due to the denaturation of the enzymes at low or high pH values. The highest production of enzymes occurred at pH values between 5.0 and 6.0, apart from the production of hyaluronate lyase of (pH 6.0 to 7.0) and the proteinase of and (pH 5.0 to 7.5). produced a lipase, hyaluronate lyase, phosphatase and proteinase activity. produced a lipase and proteinase activity.


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