1887

Abstract

CBS 621 was grown in chemostat cultures at = 0.1 h on glucose, xylose, gluconate, acetate, or ethanol as the growth-limiting substrate with ammonia or nitrate as the nitrogen source and analysed for NADPH-producing and NADPH-consuming enzyme activities.

Nitrate and nitrite reductases were strictly NADPH-dependent. For all carbon sources, growth with nitrate resulted in elevated levels of HMP pathway enzymes. NADP-linked isocitrate dehydrogenase did not vary significantly with the NADPH requirement for biosynthesis. Growth on ethanol strongly enhanced activity of NADP-linked aldehyde dehydrogenase. Neither NADP-linked malic enzyme nor transhydrogenase activities were detectable under any of the growth conditions. The absence of transhydrogenase was confirmed by the enzyme profiles of cells grown on mixtures of glucose and formate.

It is concluded that the HMP pathway and possibly NADP-linked isocitrate dehydrogenase are the major sources of NADPH in

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1983-04-01
2024-04-20
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References

  1. Bruinenberg P. M., Van Dijken J. P., Scheffers W. A. 1982; Bildung und Verbrauch von NADPH während des Wachstums von Candida utilis auf Xylose. Abstracts 5. Symposium Technische Mikrobiologie, Institut für Garungsgewerbe und Biotechnologie, Berlin.
    [Google Scholar]
  2. Bruinenberg P. M., Van Dijken J. P., Scheffers W. A. 1983; A theoretical analysis of NADPH production and consumption in yeasts. Journal of General Microbiology 129:953–964
    [Google Scholar]
  3. Burn V. J., Turner P. R., Brown C. M. 1974; Aspects of inorganic nitrogen assimilation in yeasts. Antonie van Leeuwenhoek 40:93–102
    [Google Scholar]
  4. Gancedo J. M., Gancedo C. 1971; Fructose-1,6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and nonfermenting yeasts. Archiv für Mikrobiologie 16:132–138
    [Google Scholar]
  5. Gancedo J. M., Lagunas R. 1973; Contribution of the pentose-phosphate pathway to glucose metabolism in Saccharomyces cerevisiae: a critical analysis on the use of labelled glucose. Plant Science Letters 1:193–200
    [Google Scholar]
  6. Hankinson O., Cove P. J. 1974; Regulation of the pentose phosphate pathway in the fungus Aspergillus nidulans. Journal of Biological Chemistry 249:2344–2353
    [Google Scholar]
  7. Hirai M., Shiotani T., Tanaka A., Fukui S. 1976; Effects of carbon and nitrogen sources on the levels of several NADP- and NAD-linked dehydrogenase activities of hydrocarbon-utilizable Candida yeasts. Agricultural and Biological Chemistry 40:1819–1827
    [Google Scholar]
  8. Hult K., Veide A., Gatenbeck S. 1980; The distribution of the NADPH regenerating mannitol cycle among fungal species. Archives of Microbiology 128:253–255
    [Google Scholar]
  9. Krul J. 1975 A study on the NAD(P)+transhydrogenase from Azotobacter vinelandii. Ph.D. thesis, Landbouw Hogeschool Wageningen
    [Google Scholar]
  10. Lagunas R., Gancedo J. M. 1973; Reduced pyridine-nucleotides balance in glucose-growing Saccharomyces cerevisiae. European Journal of Biochemistry 37:90–94
    [Google Scholar]
  11. Osmond C. B., Ap Rees T. 1969; Control of the pentose-phosphate pathway in yeast. Biochimica et biophysica acta 184:35–42
    [Google Scholar]
  12. Suomalainen H., Oura E. 1971; Yeast nutrition and solute uptake. In The Yeasts 2 pp. 3–74 Edited by Rose A. H., Harrison J. S. London & New York: Academic Press;
    [Google Scholar]
  13. Zwart K., Veenhuis M., Van Dijken , Harder W. 1980; Development of amine oxidase-containing peroxisomes in yeasts during growth on glucose in the presence of methylamine as the sole source of nitrogen. Archives of Microbiology 126:117–126
    [Google Scholar]
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