1887

Abstract

39–5 is a haemolytic, bacteriocinogenic strain harbouring six plasmids. One of these plasmids, pPD1 (36·4 MDal) determines a bacteriocin and encodes a conjugative response to the sex pheromone cPD1 excreted by recipient (plasmid-free) strains. The pheromone response is characterized by the formation of mating aggregates of donors (responders) with recipients. Aggregation required the presence of phosphate and divalent cations and was inhibited by agents or conditions that destroy protein structure. Aggregation was postulated to be due to synthesis of a new proteinaceous molecule on the donor cell surface. Referred to as ‘aggregation substance’, such a material was identified and found to exhibit antigenic properties not associated with uninduced cells; it could be detected by immunoelec-tron microscopy. Aggregation substance could be extracted from induced cells but not uninduced cells as demonstrated by crossed immunoelectrophoresis. Antibody raised against the aggregation substance controlled by pPD1 cross-reacted with aggregation substance determined by other plasmid systems which respond to pheromones unrelated to cPD1.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-4-1207
1983-04-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/4/mic-129-4-1207.html?itemId=/content/journal/micro/10.1099/00221287-129-4-1207&mimeType=html&fmt=ahah

References

  1. Brock T. D., Davie J. M. 1963; Probable identity of a group D hemolysin with a bacteriocin. Journal of Bacteriology 86:708–712
    [Google Scholar]
  2. Clarke H., Freeman T. A. 1967; A quantitative immunoelectrophoresis method. In Protides of the Biological Fluids 14 pp. 503–509 Peters H. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  3. Clewell D. B. 1981; Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiological Reviews 45:409–436
    [Google Scholar]
  4. Clewell D. B., Brown B. 1980; Sex pheromone cADl in Streptococcus faecalis: induction of a function related to plasmid transfer. Journal of Bacteriology 143:1063–1065
    [Google Scholar]
  5. Clewell D. B., Yagi Y., Bauer B. 1975; Plasmid determined tetracycline resistance in Streptococcus faecalis. Evidence for gene amplification during growth in the presence of tetracycline. Proceedings of the National Academy of Sciences of the United States of America 72:1720–1724
    [Google Scholar]
  6. Clewell D. B., Yagi Y., Ike Y., Craig R. A., Brown B. L., An F. 1982; Sex pheromones in Streptococcus faecalis. Multiple pheromone systems in strain DS5, similarities of pADl and pAMyl, and mutants of pADl altered in conjugative properties. In Microbiology 1982 pp. 97–100 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  7. Dunny G. 1978 Gene transfer in Streptococcus faecalis: conjugation, mating aggregates, and sex pheromones. Ph.D. thesis University of Michigan, U.S.A.:
    [Google Scholar]
  8. Dunny G., Clewell D. 1975; Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid. Journal of Bacteriology 124:784–790
    [Google Scholar]
  9. Dunny G., Brown B., Clewell D. B. 1978; Induced cell aggregation and mating in Streptococcus faecalis. Evidence for a bacterial sex pheromone. Proceedings of the National Academy of Sciences of the United States of America 75:3479–3483
    [Google Scholar]
  10. Dunny G. M., Craig R., Carron R., Clewell D. B. 1979; Plasmid transfer in Streptococcus faecalis. Production of multiple sex pheromones by recipients. Plasmid 2:454–465
    [Google Scholar]
  11. Franke A. 1980 A tetracycline resistance transposon (Tn916) in Streptococcus faecalis: transposition and conjugal transfer. Ph.D. thesis University of Michigan, U.S.A.:
    [Google Scholar]
  12. Franke A., Clewell D. B. 1981; Evidence for a chromosome-borne resistance transposon in Streptococcus faecalis capable of “conjugal” transfer in the absence of a conjugative plasmid. Journal of Bacteriology 145:494–502
    [Google Scholar]
  13. Granato P. A., Jackson R. W. 1969; Biocomponent nature of lysin from Streptococcus zymo- genes. Journal of Bacteriology 100:865–868
    [Google Scholar]
  14. Harboe N., Ingild A. 1973; Immunization, isolation of immuno-globulins, estimation of antibody titer. Scandinavian Journal of Immunology 2: Suppl.1 161–164
    [Google Scholar]
  15. Jacob A., Hobbs S. J. 1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var.zymogenes. Journal of Bacteriology 117:360–372
    [Google Scholar]
  16. Jones G. W. 1977; The attachment of bacteria to the surfaces of animal cells. In Microbial Interactions, Series B. Receptors and Recognition 3 pp. 139–176 Reissig J. L. Edited by London: Chapman & Hall;
    [Google Scholar]
  17. Kessler R. E., Yagi Y., Clewell D. B. 1982; Identification and partial purification of a surface antigen of Streptococcus faecalis that is synthesized during pheromone-induced aggregation. In Microbiology 1982 pp. 101–102 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  18. Lai C., Listgarten M. A., Rosan B. 1975; Immunoelectron microscopic identification and localization of Streptococcus sanguis with peroxidase- labeled antibody: localization of surface antigens in pure cultures. Infection and Immunity 2:193–199
    [Google Scholar]
  19. Laurell C. 1965; Antigen-antibody cross immunoelectrophoresis. Analytical Biochemistry 10:358–361
    [Google Scholar]
  20. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., Mccowan S. M. 1978; A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1:417–420
    [Google Scholar]
  21. Rosan B., Williams N. 1964; Hyaluronidase production by oral enterococci. Archives of Oral Biology 9:291–298
    [Google Scholar]
  22. Tomich P., An F., Damle S., Clewell D. 1979; Plasmid-related transmissibility and multiple drug resistance in Streptococcus faecalis subsp.zymogenes strain DS16. Antimicrobial Agents and Chemotherapy- 15:828–830
    [Google Scholar]
  23. Tomich P., An F., Clewell D. B. 1980; Properties of erythromycin-inducible transposon Tn9/7 in Streptococcus faecalis. Journal of Bacteriology 141:1366–1374
    [Google Scholar]
  24. Weeke B. 1973; Crossed immunoelectrophoresis. Scandinavian Journal of Immunology 2: Suppl. 1 47–56
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-4-1207
Loading
/content/journal/micro/10.1099/00221287-129-4-1207
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error