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To identify proteins synthesized during blastospore to germ tube transformation in Candida albicans, membrane and cytoplasmic protein fractions labelled with 14C were analysed by SDS-PAGE and autoradiography. Four cytoplasmic proteins were detected in pulse-labelled lysates prepared from cells forming blastospores and germ tubes at 37 °C, but not in pulse-labelled lysates prepared from cells forming blastospores at 24·5 °C. Three proteins detectable in 24·5 °C lysates were diminished in 37 °C lysates.
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