1887

Abstract

Antisera raised against purified yeast ascospores caused agglutination of both ascospores and vegetative cells. A spore-specific activity was obtained by absorbing out anti-vegetative activity with vegetative cells. The anti-vegetative cell activity was directed against mannan, and was probably due to exposure of some spore coat mannan at the spore surface since concanavalin A and lentil lectin also caused agglutination of ascospores. The spore-specific activity was probably determined by a protein or proteins, since extraction of spores with a mixture of sodium dodecyl sulphate and dithiothreitol markedly affected their agglutination by the spore-specific serum.

The spore-specific antigen was synthesized in a soluble form during sporulation several hours before the appearance of the spore surface and the pool of soluble antigen declined as the spore was assembled. Synthesis of the soluble antigen was inhibited by adding cycloheximide at all times up to its first appearance in the sporulating cell.

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/content/journal/micro/10.1099/00221287-129-4-1103
1983-04-01
2024-03-29
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References

  1. Ballou C. 1976; Structure and biosynthesis of the mannan component of the yeast cell envelope. Advances in Microbial Physiology 14:93–158
    [Google Scholar]
  2. Beckett A., Illingworth R. F., Rose A. H. 1973; Ascospore wall developments in Saccharomyces cerevisiae. Journal of Bacteriology 113:1054–1057
    [Google Scholar]
  3. Briley M. S., Illingworth R. F., Rose A. H., Fisher D. J. 1970; Evidence for a surface protein layer on the Saccharomyces cerevisiae ascospore. Journal of Bacteriology 104:588–589
    [Google Scholar]
  4. Clancy M. J., Smith L. M., Magee P. T. 1982; Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae. Molecular and Cellular Biology 2:171–178
    [Google Scholar]
  5. Dawes I. W., Wright J. F., Vezinhet F., Ajam N. 1980; Separation on Urografin gradients of subpopulations from sporulating Saccharomyces cerevisiae cultures. Journal of General Microbiology 119:165–171
    [Google Scholar]
  6. Esposito R. E., Klapholz S. 1981; Meiosis and ascospore development. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance pp. 211–287 Strathern J. N., Jones E. W., Broach J. R. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  7. Esposito R. E., Frink N., Bernstein P., Esposito M. S. 1972; The genetic control of sporulation in Saccharomyces. II. Dominance and complementation of mutants of meiosis and spore formation. Molecular and General Genetics 114:241–248
    [Google Scholar]
  8. Fast D. 1973; Sporulation synchrony of Saccharomyces cerevisiae in various carbon sources. Journal of Bacteriology 116:925–930
    [Google Scholar]
  9. Guth E., Hashimoto T., Conti S. 1972; Morphogenesis of ascospores in Saccharomyces cerevisiae. Journal of Bacteriology 109:869–880
    [Google Scholar]
  10. Jenkinson H. F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  11. Moens P. B. 1971; Fine structure of ascospore development in the yeast Saccharomyces cerevisiae. Canadian Journal of Microbiology 17:507–510
    [Google Scholar]
  12. Moens P. B., Rapport E. 1971; Spindles, spindle plaques and meiosis in the yeast Saccharomyces cerevisiae (Hansen). Journal of Cell Biology 50:344–361
    [Google Scholar]
  13. Muñoz L. E., Nakayama T., Doi R. H. 1978; Expression of spore coat protein gene, an “early sporulation gene”, and its relationship to RNA polymerase modification. In Spores VII pp. 213–219 Chambliss G., Vary J. C. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  14. Pandey N. K., Aronson A. I. 1979; Properties of the Bacillus subtilis spore coat. Journal of Bacteriology 137:1208–1218
    [Google Scholar]
  15. Snider I. I., Miller J. J. 1966; A serological comparison of vegetative cell and ascus wall and the spore coat of Saccharomyces cerevisiae. Canadian Journal of Microbiology 12:485–488
    [Google Scholar]
  16. Vezinhet F., Kinnaird J. H., Dawes I. W. 1979; The physiology of mutants derepressed for sporulation in Saccharomyces cerevisiae. Journal of General Microbiology 115:391–402
    [Google Scholar]
  17. Wood D. A. 1972; Sporulation in Bacillus subtilis. Properties and time of synthesis of alkali-soluble protein of the spore coat. Biochemical Journal 130:505–514
    [Google Scholar]
  18. Wright J. F., Ajam N., Dawes I. W. 1981; Nature and timing of some sporulation-specific protein changes in Saccharomyces cerevisiae. Molecular and Cellular Biology 1:910–918
    [Google Scholar]
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