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Phenazine production by Pseudomonas phenazinium was not reduced by high concentrations of phosphate during batch culture (cf. Pseudomonas aeruginosa) but was inhibited by high concentrations of (NH4)2SO4 (1 to 10 g 1−1). In carbon-, sulphate-, magnesium- and potassium-depleted medium phenazine production paralleled growth. In continuous culture, phenazine production occurred over a wide range of growth rates under various nutrient-limiting conditions. At low growth rates cell resources were conserved and cell mass was maintained at the expense of secondary metabolite production. Low oxygen tensions and the addition of phenylalanine (0.1 g 1−1) each inhibited phenazine production.
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