1887

Abstract

A simple method of isolating bacteria that utilize cyanide as a source of nitrogen for growth has been developed. This involved supplying hydrogen cyanide as a vapour to glucose-containing minimal-salts agar plates. The bacteria isolated were Gram-negative, oxidase-positive rods producing a fluorescent green pigment and were tentatively identified as strains of Three organisms were studied further and shown to be biotype II. One of these (NCIB 11764) was grown in a glucose-containing fed-batch culture with either NHCl or KCN as the limiting nutrient. Cyanide-grown bacteria produced stoichiometric amounts of ammonia from cyanide when pulsed with cyanide under aerobic conditions. Stimulation of oxygen uptake was seen on addition of cyanide to suspensions of cyanide-grown but not ammonia-grown bacteria.

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/content/journal/micro/10.1099/00221287-129-4-1005
1983-04-01
2021-07-31
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References

  1. Appleby C. A. 1969; Inhibitors of respiratory enzymes, photosynthesis and phosphorylation; uncoupling reagents. In Data for Biochemical Research, 2nd edn. pp. 380–387 Edited by Dawson R. M. C., Elliott D. C., Elliott W. H., Jones K. M. Oxford: Clarendon Press;
    [Google Scholar]
  2. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. Journal of General Microbiology 23:457–469
    [Google Scholar]
  3. Brown C. M., Macdonald-Brown D. S., Stanley S. O. 1973; The mechanisms of nitrogen assimilation in pseudomonads. Antonie van Leeuwenhoek 39:89–98
    [Google Scholar]
  4. Castric P. A. 1981; The metabolism of hydrogen cyanide by bacteria. In Cyanide in Biology pp 233–261 Edited by Vennesland B., Conn E. E., Knowles C. J., Westley J., Wissing F. London and New York: Academic Press;
    [Google Scholar]
  5. Cowan S. T. 1974; Cowan and Steel’s Manual for the Identification of Medical Bacteria. , 2nd edn.. Cambridge: Cambridge University Press;
    [Google Scholar]
  6. Drew S. W. 1981; Liquid culture. In Manual of Methods for General Bacteriology pp. 151–178 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  7. Fawcett J. K., Scott J. E. 1960; A rapid and precise method for the determination of urea. Journal of Clinical Pathology 13:156–160
    [Google Scholar]
  8. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyo- cyanin and fluorescin. Journal of Laboratory and Clinical Medicine 44:301–307
    [Google Scholar]
  9. Knowles C. J. 1976; Microorganisms and cyanide. Bacteriological Reviews 40:652–680
    [Google Scholar]
  10. Kun E., Kearney E. B. 1974; Enzymatic determination of ammonia. In Methods of Enzymatic Analysis, 2nd English edn. 4 pp. 1802–1806 Edited by Bergmeyer H. U. New York and London: Academic Press;
    [Google Scholar]
  11. Lambert J. L., Ramasamy J., ;Paukstelis J. V. 1975; Stable reagents for the colorimetric determination of cyanide by modified König reactions. Analytical Chemistry 47:916–918
    [Google Scholar]
  12. Miller J. H. 1972 Experiments in Molecular Genetics p. 431 Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Peterson E. A. 1970; Cellulosic ion exchangers. In Laboratory Techniques in Biochemistry and Molecular Biology 2 part II pp. 327–354 Edited by Work T. S., Work E. Amsterdam: North Holland/Elsevier;
    [Google Scholar]
  14. Pettet A. E. J., ;Ware G. C. 1955; Disposal of cyanide wastes. Chemistry and Industry1232–1238
    [Google Scholar]
  15. Solano-Muñoz F., Bardsley W. G. 1981; The generation of non-linear solute gradients for chromatography by using only simple apparatus. Biochemical Journal 193:991–996
    [Google Scholar]
  16. Ware G. C., Painter H. A. 1955; Bacterial utilization of cyanide. Nature, London 75:900
    [Google Scholar]
  17. Winter J. A. 1963; The use of a specific actino- mycete to degrade cyanide wastes. In Proceedings of the 18th Industrial Waste Conference pp. 703–716 Indiana: Purdue University;
    [Google Scholar]
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