Peptidoglycan-degrading Enzymes in Ether-treated Cells of Free

Abstract

The incubation of peptidoglycan fragments with ether-treated cells of resulted in breakdown products that showed the presence of previously undescribed lytic enzymes. The properties of an endopeptidase able to hydrolyse peptide-linked bis-disaccharide peptide dimer to monomer units were examined. An -acetyl-glucosaminidase was also shown to release free -acetylglucosamine. The breakdown pattern of glycosidically-linked dimer indicated the existence of an -acetylglucosaminidase. The activities of the latter enzyme and of the endopeptidase were both sensitive to lactam antibiotics.

Erratum

An erratum has been published for this content:
Peptidoglycan-degrading Enzymes in Ether-treated Cells of
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-877
1983-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-877.html?itemId=/content/journal/micro/10.1099/00221287-129-3-877&mimeType=html&fmt=ahah

References

  1. Blumberg P. M., Strominger J. L. 1974; Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes. Bacteriological Reviews 38:291–335
    [Google Scholar]
  2. Blundell J. K., Perkins H. R. 1981; Effects of β-lactam antibiotics on peptidoglycan synthesis in growing Neisseria gonorrhoeae, including changes in the degree of O-acetylation. Journal of Bacteriology 147:633–641
    [Google Scholar]
  3. Blundell J. K., Smith G. J., Perkins H. R. 1980; The peptidoglycan of Neisseria gonorrhoeae : O-acetyl groups and lysozyme sensitivity. FEMS Microbiology Letters 9:259–261
    [Google Scholar]
  4. Brown C. A., Perkins H. R. 1979; In vitro synthesis of peptidoglycan by β-lactam-sensitive and -resistant strains of Neisseria gonorrhoeae. Effects of β-lactam and other antibiotics. Antimicrobial Agents and Chemotherapy 16:28–36
    [Google Scholar]
  5. Davis R. H., Salton M. R. J. 1975; Some properties of a d-alanine carboxypeptidase in envelope fractions of Neisseria gonorrhoeae. Infection and Immunity 12:1065–1069
    [Google Scholar]
  6. Davis R. H., Linder R., Salton M. R. J. 1978; Solubilization and characterization of the partially purified penicillin sensitive d-alanine carboxypeptidase of Neisseria gonorrhoeae. Microbios 21:69–80
    [Google Scholar]
  7. Goodell E. W., Fazio M., Tomasz A. 1979; Effect of benzylpenicillin on the synthesis and structure of the cell envelope of Neisseria gonorrhoeae. Antimicrobial Agents and Chemotherapy 13:514–525
    [Google Scholar]
  8. Gubish E. R. Jr Chen K. C. S., Buchanan T. M. 1982; Detection of a gonococcal endo-β-N-acetyl-glucosaminidase and its peptidoglycan cleavage site. Journal of Bacteriology 151:172–176
    [Google Scholar]
  9. Guymon L. F., Walstad D. L., Sparling P. F. 1978; Cell envelope alterations in antibiotic-sensitive and -resistant strains of Neisseria gonorrhoeae. Journal of Bacteriology 136:391–401
    [Google Scholar]
  10. Hebeler B. H., Young F. E. 1976a; Chemical composition and turnover of peptidoglycan in Neisseria gonorrhoeae. Journal of Bacteriology 126:1180–1185
    [Google Scholar]
  11. Hebeler B. H., Young F. E. 1976b; Mechanism of autolysis of Neisseria gonorrhoeae. Journal of Bacteriology 126:1186–1193
    [Google Scholar]
  12. HÖltje J. V., Mirelman D., Sharon N., Schwarz U. 1975; Novel type of mureintransgly-cosylase in Escherichia coli. Journal of Bacteriology 124:1067–1076
    [Google Scholar]
  13. Keck W., Schwarz U. 1979; Escherichia coli murein-dd-endopeptidase insensitive to β-lactam antibiotics. Journal of Bacteriology 139:770–774
    [Google Scholar]
  14. Martin H. H., Gmeiner J. 1979; Modification of peptidoglycan structure by penicillin action in cell walls of Proteus mirabilis. European Journal of Biochemistry 95:487–495
    [Google Scholar]
  15. Pelzer H. 1963; Mucopeptidhydrolasen in Escherichia coli B. I. Nachweis und Wirkungsspezifität. Zeitschrift für Naturforschung 18b:950–956
    [Google Scholar]
  16. Primosigh J., Pelzer H., Maass D., Weidel W. 1961; Chemical characterization of mucopeptides released from the E. coli cell wall by enzymic action. Biochimica et biophysica acta 46:68–80
    [Google Scholar]
  17. Rosenthal R. S. 1979; Release of soluble peptidoglycan from growing gonococci hexaminidase and amidase activities. Infection and Immunity 24:869–878
    [Google Scholar]
  18. Rosenthal R. S., Wright R. M., Sinha R. K. 1980; Extent of peptide cross-linking in the peptidoglycan of Neisseria gonorrhoeae. Infection and Immunity 28:867–875
    [Google Scholar]
  19. Salton M. R. J. 1959; An improved method for the detection of N-acetyl-amino sugars on paper chromatograms. Biochimica et biophysica acta 34:308–312
    [Google Scholar]
  20. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  21. Sinha R. K., Rosenthal R. S. 1980; Release of soluble peptidoglycan from growing gonococci: demonstration of anhydro-muramyl-containing fragments. Infection and Immunity 29:914–924
    [Google Scholar]
  22. Tamura T., Imae Y., Strominger J. L. 1976; Purification to homogeneity and properties of two d-alanine carboxypeptidases I from Escherichia coli. Journal of Biological Chemistry 251:414–423
    [Google Scholar]
  23. Tipper D. J. 1968; Alkali-catalysed elimination of d-lactic acid from muramic acid and its derivatives and the determination of muramic acid. Biochemistry 7:1441–1449
    [Google Scholar]
  24. Tomioka S., Matsuhashi M. 1978; Purification of penicillin-insensitive dd-endopeptidase: a new cell wall peptidoglycan-hydrolysing enzyme in Escherichia coli,and its inhibition by deoxyribonucleic acids. Biochemical and Biophysical Research Communications 84:978–984
    [Google Scholar]
  25. Ward J. B. 1973; The chain length of the glycans in bacterial cell walls. Biochemical Journal 133:395–398
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-877
Loading
/content/journal/micro/10.1099/00221287-129-3-877
Loading

Data & Media loading...

Most cited Most Cited RSS feed