1887

Abstract

Experimental conditions which allowed liquid suspensions of lupin bacteroids ( NZP 2257) to reduce acetylene at high rates were established. At a dissolved oxygen concentration of 2 μ, swirled suspensions of the bacteroids reduced acetylene at a linear rate of 23 ± 5 nmol (mg cell protein) min. The kinetics of protein synthesis were measured by pulse labelling with [S]methionine followed by two-dimensional electrophoretic analysis of cell lysates. When the suspensions were suddenly exposed to air, the rate of synthesis of the two nitrogenase components declined differentially. The decline in the rate of synthesis of the Fe-protein polypeptides resembled that observed for all the nitrogenase polypeptides in ( Eady ., 1978 ); the rate of synthesis of the MoFe-protein polypeptides, however, showed a much slower decline. Several models are proposed to account for these results.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-849
1983-03-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-849.html?itemId=/content/journal/micro/10.1099/00221287-129-3-849&mimeType=html&fmt=ahah

References

  1. Bergersen F. J. 1966; Some properties of nitrogenfixing breis prepared from soybean root nodules. Biochimica et biophysica acta 130:304–312
    [Google Scholar]
  2. Bergersen F. J., Turner G. L. 1975; Leghaemo- globin and the supply of O2 to nitrogen-fixing root nodule bacteroids: studies of an experimental system with no gas phase. Journal of General Microbiology 89:31–47
    [Google Scholar]
  3. Bisseling T., Van Den Bos R. C., Van Kammen A. 1978; The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced byRhizobium leguminosarum. . Biochimica et biophysica acta 539:1–11
    [Google Scholar]
  4. Bisseling T., Van Den Bos R. C., Weststrate M. W., Hakkaart M. J. J., Van Kammen A. 1979; Development of the nitrogen-fixing and protein- synthesizing apparatus of bacteroids in pea root nodules. Biochimica et biophysica acta 562:515–526
    [Google Scholar]
  5. Bisseling T., Van Staveren T., Van Kammen A. 1980a; The effect of waterlogging on the synthesis of the nitrogenase components in bacteroids ofRhizobium leguminosarum in root nodules ofPisum sativum. . Biochemical and Biophysical Research Communications 93:687–693
    [Google Scholar]
  6. Bisseling T., Moen A. A., Van Den Bos R. C., Van Kammen A. 1980b; The sequence of appearance of leghaemoglobin and nitrogenase components I and II in root nodules ofPisum sativum. . Journal of General Microbiology 118:377–381
    [Google Scholar]
  7. Dalton H. 1980; The cultivation of diazotrophic micro-organisms. In Methods for Evaluating Biological Nitrogen Fixation pp. 13–64 Edited by Bergersen F. J. Chichester: John Wiley;
    [Google Scholar]
  8. Dixon R., Eady R. R., Espin G., Hill S., Iaccarino M., Kahn D., Merrick M. 1980; Analysis of regulation ofKlebsiella pneumoniaenitrogen fixation(nif) gene cluster with gene fusions. Nature, London 286:128–132
    [Google Scholar]
  9. Eady R. R., Issack R., Kennedy C., Postgate J. R., Ratcliffe H. D. 1978; Nitrogenase synthesis inKlebsiella pneumoniae: comparison of ammonium and oxygen regulation. Journal of General Microbiology 104:277–285
    [Google Scholar]
  10. Harker A. R., Wullstein L. H. 1981; Resolution of two subunits from the molybdenum-iron protein ofAzotobacter vinelandii nitrogenase. Journal of Biological Chemistry 256:11981–11983
    [Google Scholar]
  11. Hennecke H. 1981; Recombinant plasmids carrying nitrogen fixation genes fromRhizobium japonicum. . Nature, London 291:354–355
    [Google Scholar]
  12. Hill S., Kennedy C., Kavanagh E., Goldberg R. B., Hanau R. 1981; Nitrogen fixation gene(nifL)involved in oxygen regulation of nitrogenase synthesis inK. . pneumoniae Nature, London 290:424–426
    [Google Scholar]
  13. Kennedy C., Eady R. R., Kondorosi E., Rekosh D. K. 1976; The molybdenum-iron protein ofKlebsiella pneumoniae nitrogenase: evidence for nonidentical subunits from peptide ‘mapping’. Biochemical Journal 155:383–389
    [Google Scholar]
  14. Laane C., Haaker H., Veeger C. 1978; Involvement of the cytoplasmic membrane in nitrogen fixation byRhizobium leguminosarum bacteroids. European Journal of Biochemistry 87:147–153
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. O’FARRELL P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  17. Roberts G. P., Macneil T., Macneil D., Brill W. J. 1978; Regulation and characterization of protein products coded by thenif (nitrogen fixation) genes ofKlebsiella pneumoniae. . Journal of Bacteriology 136:267–279
    [Google Scholar]
  18. Robertson J. G., Warburton M. P., Lyttleton P., Fordyce A. M., Bullivant S. 1978; Membranes in lupin root nodules. II. Preparation and properties of peribacteroid membranes and bacter- oid envelope inner membranes from developing lupin nodules. Journal of Cell Science 30:151–174
    [Google Scholar]
  19. Robson R. L. 1979; O2-repression of nitrogenase synthesis inAzotobacter chroococcum. . FEMS Microbiology Letters 5:259–262
    [Google Scholar]
  20. Ruvkun G. B., Ausubel F. M. 1980; Interspecies homology of nitrogenase genes. Proceedings of the National Academy of Sciences of the United States of America 77:191–195
    [Google Scholar]
  21. Scott D. B., Hennecke H., Lim S. T. 1979; The biosynthesis of nitrogenase MoFe protein polypeptides in free-living cultures ofRhizobium japonicum. . Biochimica et biophysica acta 565:365–378
    [Google Scholar]
  22. Shah V. K. 1980; Isolation of the iron-molybdenum cofactor from nitrogenase. Methods in Enzymology 69:792–801
    [Google Scholar]
  23. Shaw B. D. 1981; Lupin bacteroids shocked by oxygen. In Current Perspectives in Nitrogen Fixation p. 446 Edited by Gibson A. H., Newton W. E. Canberra: Australian Academy of Science;
    [Google Scholar]
  24. Sutton W. D., Mahoney P. 1977; Preparation and fractionation ofRhizobium bacteroids by zone sedimentation through sucrose gradients. Plant Physiology 60:800–802
    [Google Scholar]
  25. Whiting M. J., Dilworth M. J. 1974; Legume root nodule nitrogenase: purification, properties, and studies on its genetic control. Biochimica et biophysica acta 371:337–351
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-849
Loading
/content/journal/micro/10.1099/00221287-129-3-849
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error