1887

Abstract

Phenols are able to lower the temperature of heat activation (breaking of dormancy by heat shock) in spores. The effect was observed with a series of phenols ranging, according to increasing lipophilic character, from unsubstituted phenol to 2,4-dichlorophenol. However, the concentration required to produce the same effect with each phenol was reduced with increasing apolar character. A linear relationship was obtained between the log of the concentration of each phenol needed to produce a 4 °C shift of the half-activation temperature and the log of its octanol/water partition coefficient. In contrast, the isopropyl-substituted phenols thymol, 2- and 4-isopropylphenol and 3-isopropylcatechol all raised the half-activation temperature of the spores. The same effect was observed with menthol, the unsaturated analogue of thymol. The heat resistance of the spores was lowered by all phenols, including isopropyl-substituted phenols. Although the reason for the anomalous behaviour of isopropyl-substituted phenols is not known, the opposite effect on spore heat activation and spore heat resistance indicates that the activation process of the spores is not linked to the process of spore killing. Therefore, spore activation is not due to some kind of non-specific sublethal protein denaturation, as might have been concluded previously from the fact that many spore activation methods are sublethal treatments.

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1983-03-01
2021-10-20
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References

  1. Borchert R. 1962; Über die Azetat-Aktivierung der Sporangiosporen von Phycomyces blakesleeanus. . Bei-träge zur Biologie der Pflanzen 38:31–61
    [Google Scholar]
  2. Cotter D. A. 1979; Activation of Dictyostelium discoideum spores with guanidine and methyl derivatives of urea. Current Microbiology 2:27–30
    [Google Scholar]
  3. Cotter D. A., George R. P. 1975; Germination and mitochondrial damage in spores of Dictyostelium discoideum following supraoptimal heating. Archives of Microbiology 103:163–168
    [Google Scholar]
  4. Cotter D. A., O’Connell R. W. 1976; Activation and killing of Dictyostelium discoideum spores with urea. Canadian Journal of Microbiology 22:1751–1755
    [Google Scholar]
  5. Cotter D. A., Raper K. B. 1966; Spore germination in Dictyostelium discoideum. . Proceedings of the National Academy of Sciences of the United States of America 56:880–887
    [Google Scholar]
  6. Cotter D. A., Morin J. W., O’Connell R. W. 1976; Spore germination in Dictyostelium discoideum. II. Effects of dimethyl sulfoxide on postactivation lag as evidence for the multistate model of activation. Archives of Microbiology 108:93–98
    [Google Scholar]
  7. Dobbs C. G., Hinson W. H. 1953; A widespread fungistasis in soils. Nature; London: 172197–199
    [Google Scholar]
  8. Emerson M. R. 1954; Physiological characteristics of ascospore activation in Neurospora crassa. . Plant Physiology 29:418–428
    [Google Scholar]
  9. Goddard D. R. 1935; The reversible heat activation inducing germination and increased respiration in the ascospores of Neurospora tetrasperma. . Journal of General Physiology 19:45–60
    [Google Scholar]
  10. Hansch C., Dunn W. J. 1972; Linear relationship between lipophilic character and biological activity of drugs. Journal of Pharmaceutical Sciences 61:1–19
    [Google Scholar]
  11. Hashimoto Y. 1971; Effect of radiation on the germination of Dictyostelium discoideum. . Nature; London: 231316–317
    [Google Scholar]
  12. Hashimoto Y., Yanagisawa K. 1970; Effect of radiation on the spore germination of the cellular slime mold Dictyostelium discoideum. . Radiation Research 44:649–659
    [Google Scholar]
  13. Khoury A. T., Deering R. A., Levin G., Altman G. 1970; Gamma-ray-induced spore germination of Dictyostelium discoideum. . Journal of Bacteriology 104:1022–1023
    [Google Scholar]
  14. Mills G. L., Eilers F. I. 1973; Factors influencing the germination of basidiospores of Coprinus radiatus. . Journal of General Microbiology 77:393–401
    [Google Scholar]
  15. Rudolph H. 1958; Entwicklungsphysiologische Untersuchungen an den Sporangiophoren von Phycomyces blakesleeanus. . Biologisches Zentralblatt 77:385–437
    [Google Scholar]
  16. Schneider H. 1968; The intramembrane location of alcohol anesthetics. Biochimica et biophysica acta 163:451–458
    [Google Scholar]
  17. Seeman P. 1972; The membrane actions of anesthetics and tranquilizers. Pharmacological Reviews 24:583–655
    [Google Scholar]
  18. Sommer L., Halbsguth W. 1957; Grundlegende Versuche zur Keimungsphysiologie von Pilzsporen. Forschungsberichte des Wirtschafts-und Verkehrs-ministeriums Nordrhein-Westfalen 411:
    [Google Scholar]
  19. Sussman A. S. 1953; The effect of heterocyclics and other compounds upon the germination of Neurospora tetrasperma. . Journal of General Microbiology 8:211–216
    [Google Scholar]
  20. Sussman A. S. 1965; Physiology of dormancy and germination in the propagules of cryptogamic plants. Handbuch der Pflanzenphysiologie XV(2:933–1025
    [Google Scholar]
  21. Sussman A. S. 1976; Activators of fungal spore germination. In The Fungal Spore pp. 101–139 Weber D. J., Hess W. M. Edited by New York: John Wiley.;
    [Google Scholar]
  22. Sussman A. S., Lowry R. J., Tyrrell E. 1959; Activation of Neurospora ascospores by organic solvents and furans. Mycologia 51:237–247
    [Google Scholar]
  23. Thevelein J. M., Vanassche J. A., Carlier A. R., Heremans K. 1979; Heat activation of Phycomyces blakesleeanus spores: thermodynamics and effect of alcohols, furfural and high pressure. Journal of Bacteriology 139:478–485
    [Google Scholar]
  24. Thevelein J. M., Vanassche J. A., Heremans K., Gerlsma S. Y., Carlier A. R. 1981; Trehalase activity in extracts of Phycomyces blakesleeanusspores following the induction of germination by heat activation. Antonie van Leeuwenhoek 47:393–404
    [Google Scholar]
  25. Vanassche J. A., Carlier A. R., Dekeersmaeker H. I. 1972; Trehalase activity in dormant and activated spores of Phycomyces blakesleeanus. . Planta 103:327–333
    [Google Scholar]
  26. Van Assche J. A., Carlier A. R., Van Tieghem L. L. C. 1977; The effect of gamma radiation on breaking of dormancy in Phycomyces spores. Archives of Microbiology 113:95–97
    [Google Scholar]
  27. Van Assche J. A., Van Laere A. J., Carlier A. R. 1978; Trehalose metabolism in dormant and activated spores of Phycomyces blakesleeanus Bur-geff. Planta 139:171–176
    [Google Scholar]
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