1887

Abstract

The respiratory capacity of growing in continuous culture on glucose and on mixtures of glucose and ethanol was investigated. An oxygen uptake rate of 8 mmo1 g h was found to limit the ability of the organism to degrade a substrate purely oxidatively. On glucose as sole energy and carbon source, this respiration rate was invariably achieved at an identical growth rate and thus at an identical substrate uptake rate when the inlet glucose concentration was varied. The rate of ethanol co-consumption together with glucose was strictly governed by this limiting maximum respiratory capacity and no repression of respiration was observed at dilution rates where ethanol was excreted by the cells. Hence, a limitation in some step in the oxidative branch of catabolism is likely to be responsible for incomplete oxidation of glucose at high growth rates rather than an undefined action of glucose repression.

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/content/journal/micro/10.1099/00221287-129-3-653
1983-03-01
2021-07-25
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References

  1. Barford J. P., Hall R. J. 1979a; Investigation of the significance of a carbon and redox balance to the measurement of gaseous metabolism in Saccharomyces cerevisiae. Biotechnology and Bioengineering 21:609–626
    [Google Scholar]
  2. Barford J. P., Hall R. J. 1979b; An examination of the Crabtree Effect in Saccharomyces cerevisiae: the role of respiratory adaptation. Journal of General Microbiology 114:267–275
    [Google Scholar]
  3. Beck C., Von Meyenburg H. K. 1968; Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation. Journal of Bacteriology 96:479–486
    [Google Scholar]
  4. Bijkerk A. H. E., Hall R. J. 1977; A mechanistic model for the aerobic growth of Saccharomyces cerevisiae. Biotechnology and Bioengineering 19:267–296
    [Google Scholar]
  5. Ciriacy M., Breitenbach I. 1979; Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae. Journal of Bacteriology 139:152–160
    [Google Scholar]
  6. Crabtree H. G. 1929; Observations on the carbohydrate metabolism of tumors. Biochemical Journal 23:536–545
    [Google Scholar]
  7. De Deken R. H. 1966; The Crabtree Effect.A regulatory system in yeast. Journal of General Microbiology 44:149–156
    [Google Scholar]
  8. Entian K. D. 1977; Lack of carbon catabolite inactivation in a mutant of Saccharomyces cerevisiae with reduced hexokinase activity. Molecular and General Genetics 158:201–210
    [Google Scholar]
  9. Fiechter A., Fuhrmann G. F., Käppeli O. 1981; Regulation of glucose metabolism in growing yeast cells. Advances in Microbial Physiology 22:123–183
    [Google Scholar]
  10. Geurts TH. G. E., De Kok H. E., Roels J. A. 1980; A quantitative description of the growth of Saccharomyces cerevisiae CBS 426 on a mixed substrate of glucose and ethanol. Biotechnology and Bioengineering 22:2031–2043
    [Google Scholar]
  11. Haarasilta S., Oura E. 1975; On the activity regulation of anaplerotic and gluconeogenetic enzymes during the growth phase of baker’s yeast. European Journal of Biochemistry 52:1–7
    [Google Scholar]
  12. Herbert D. 1975; Stoicheiometric aspects of microbial growth. In Continuous Culture 6 pp. 1–30 Edited by Dean A. C. R., Ellwood D. C., Evans C. G. T., Melling J. Chichester: Ellis Horwood;
    [Google Scholar]
  13. Holzer H. 1976; Catabolite inactivation in yeast. Trends in Biochemical Sciences 1:178–181
    [Google Scholar]
  14. Karrer D. 1978 Der total gefullteBioreaktor. Ph.D. thesis, Eidgenössische Technische Hochschule, Zürich
    [Google Scholar]
  15. Knoepfel H. P. 1972 Zum Crabtree Effektbei Saccharomyces cerevisiae und Candida tropicalis. Ph.D. thesis, Eidgenössische Technische Hochschule, Zürich
    [Google Scholar]
  16. Meyenburg H. K., Von. 1969 Katabolit Repression und der Sprossungszyklus von Saccharomyces cerevisiae. Ph.D. thesis, Eidgenossische Technische Hochschule, Zurich
    [Google Scholar]
  17. Polakis E. S., Bartley W. 1965; Changes in enzyme activities of Saccharomyces cerevisiae during growth on different carbon sources. Biochemical Journal 97:284–297
    [Google Scholar]
  18. Polakis E. S., Bartley W., Meek J. A. 1965; Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources. Biochemical Journal 97:298–302
    [Google Scholar]
  19. Schatzmann H. 1975 Anaerobes Wachstum von Saccharomyces cerevisiae. Ph.D. thesis, Eidgenössische Technische Hochschule, Zürich
    [Google Scholar]
  20. Witt I., Kronau R., Holzer H. 1966; Repression von Alkoholdehydrogenase, Malatdehydro- genase, Isocitratlyase und Malatsynthase in Hefedurch Glucose. Biochimica et biophysica acta 118:522–537
    [Google Scholar]
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