1887

Abstract

Bioluminescence ATP analysis has been used to assess bacterial adhesion with hydrophobic polystyrene tubes as the attachment surface. The assay was performed at 37 °C and pH 6.8 with a 10 min incubation period. A variation of more than 200-fold was observed in the adherence capacity of 34 urinary isolates of and organisms could be classified as strongly or weakly adherent. All strains capable of strong adhesion possessed both type 1 fimbriae and flagella, and maximum adhesion was expressed during the exponential growth phase. Attachment was in all cases virtually eliminated by addition of 2.5% (w/v) -mannose to the incubation buffer. Conversely, strains which were deficient in type 1 fimbriae or flagella, or both, were weakly adherent during all phases of growth. There was no correlation between adherence of to polystyrene and adherence to buccal or uroepithelial cells, but there was a significant association with adherence to uromucoid ( < 0.002).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-3-621
1983-03-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/3/mic-129-3-621.html?itemId=/content/journal/micro/10.1099/00221287-129-3-621&mimeType=html&fmt=ahah

References

  1. Aronson M., Medalia O., Schori L., Mirelman D., Sharon N., Ofek I. 1979; Prevention of colonization of the urinary tract of mice with Escherichia coliby blocking of bacterial adherence with methyl α-d-mannopyranoside. Journal of Infectious Diseases 139:329–332
    [Google Scholar]
  2. Bar-Shavit Z., Goldman R., Ofek I., Sharon N., Mirelman D. 1980; Mannose-binding activity of Escherichia coli: a determinant of attachment and ingestion of the bacteria by macrophages. Infection and Immunity 29:417–424
    [Google Scholar]
  3. Brinton C. C.Jr 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram-negative bacteria. Transactions of the New York Academy of Sciences 27:1003–1054
    [Google Scholar]
  4. Chick S., Asscher A. W. 1980; Studies on the significance of bacterial adherence in experimental urinary tract infection. In Pyelonephritis, vol. IV, Urinary Tract Infections pp. 67–71 Losse H., Asscher A. W., Lison A. E. Edited by Stuttgart & New York:: Georg Thieme Verlag.;
    [Google Scholar]
  5. Chick S., Harber M. J., Mackenzie R., Asscher A. W. 1981; Modified method for studying bacterial adhesion to isolated uroepithelial cells and uromucoid. Infection and Immunity 34:256–261
    [Google Scholar]
  6. De Boer W. E., Golten C., Scheffers W. A. 1975; Effects of some physical factors on flagellation and swarming of Vibrio alginolyticus. Netherlands Journal of Sea Research 9:197–213
    [Google Scholar]
  7. Distler W., KrÖncke A., Maurer G. 1980; Adenosine triphosphate content of human dental plaque as a measure of viable cell mass. Caries Research 14:265–268
    [Google Scholar]
  8. Fader R. C., Avots-Avotins A. E., Davis C. P. 1979; Evidence for pili-mediated adherence of Klebsiella pneumoniaeto rat bladder epithelial cells in vitro. Infection and Immunity 25:729–737
    [Google Scholar]
  9. Fletcher M. 1976; The effects of proteins on bacterial attachment to polystyrene. Journal of General Microbiology 94:400–404
    [Google Scholar]
  10. Fletcher M. 1977; The effects of culture concentration and age, time, and temperature on bacterial attachment to polystyrene. Canadian Journal of Microbiology 23:1–6
    [Google Scholar]
  11. Fletcher M. 1979; The attachment of bacteria to surfaces in aquatic environments. In Adhesion of Micro-organisms to Surfaces pp. 87–108 Ellwood D. C., Melling J., Rutter P. Edited by London, New York & San Francisco:: Academic Press.;
    [Google Scholar]
  12. Guentzel M. N., Berry L. J. 1975; Motility as a virulence factor for Vibrio cholerae. Infection and Immunity 11:890–897
    [Google Scholar]
  13. Harber M. J. 1982; Applications of luminescence in medical microbiology and haematology. In Clinical and Biochemical Luminescence pp. 189–218 Kricka L. J., Carter T. J. N. Edited by New York:: Marcel Dekker.;
    [Google Scholar]
  14. Harber M. J., Chick S., Mackenzie R., Asscher A. W. 1982a; Lack of adherence to epithelial cells by freshly isolated urinary pathogens. Lancet i:586–588
    [Google Scholar]
  15. Harber M. J., Mackenzie R. K., Asscher A. W. 1982b; Bacterial adherence and the urinary tract. Lancet i:1352–1353
    [Google Scholar]
  16. Jones G. W., Richardson L. A., Uhlman D. 1981; The invasion of HeLa cells by Salmonella typhimurium: reversible and irreversible bacterial attachment and the role of bacterial motility. Journal of General Microbiology 127:351–360
    [Google Scholar]
  17. Källenius G., Möllby R., Svenson S. B., Winberg J., Hultberg H. 1980; Identification of a carbohydrate receptor recognized by uropathogenicEscherichia coli. Infection 8: (supplement 3) S288–S293
    [Google Scholar]
  18. Källenius G., Möllby R., Svenson S. B., Helin I., Hultberg H., Cedergren B., Winberg J. 1981; Occurrence of P-fimbriatedEscherichia coli in urinary tract infections. Lancet ii:1369–1372
    [Google Scholar]
  19. Leffler H., Svanborg edén C. 1980; Chemical identification of a glycosphingolipid receptor for Escherichia coli attaching to human urinary tract epithelial cells and agglutinating human erythrocytes. FEMS Microbiology Letters 8:127–134
    [Google Scholar]
  20. Mangan D. F., Snyder I. S. 1979; Mannose-sensitive interaction of Escherichia coliwith human peripheral leukocytes in vitro. Infection and Immunity 26:520–527
    [Google Scholar]
  21. Meadows P. S. 1971; The attachment of bacteria to solid surfaces. Archiv für Mikrobiologie 75:374–381
    [Google Scholar]
  22. Ørskov I., Ørskov F., Blrch-Anderson A. 1980a; Comparison of Escherichia colifimbrial antigen F7 with type 1 fimbriae. Infection and Immunity 27:657–666
    [Google Scholar]
  23. Ørskov I., Ferencz A., Ørskov F. 1980b; Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type 1 fimbriatedEscherichia coli. Lancet i:887
    [Google Scholar]
  24. Robrish S. A., Kemp C. W., Bowen W. H. 1978; Use of extractable adenosine triphosphate to estimate the viable cell mass in dental plaque samples obtained from monkeys. Applied and Environmental Microbiology 35:743–749
    [Google Scholar]
  25. Rutter P. R., Abbott A. 1978; A study of the interaction between oral streptococci and hard surfaces. Journal of General Microbiology 105:219–226
    [Google Scholar]
  26. Silverblatt F. J., Dreyer J. S., Schauer S. 1979; Effect of pili on susceptibility of Escherichia colito phagocytosis. Infection and Immunity 24:218–223
    [Google Scholar]
  27. Smyth C. J., Jonsson P., Olsson E., Söderlind O., Rosengren J., HjertÉn S., WadstrÖm T. 1978; Differences in hydrophobic surface characteristics of porcine enteropathogenicEscherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography. Infection and Immunity 22:462–472
    [Google Scholar]
  28. Tomita T., Blumenstock E., Kanegasaki S. 1981; Phagocytic and chemiluminescent responses of mouse peritoneal macrophages to living and killed Salmonella typhimurium and other bacteria. Infection and Immunity 32:1242–1248
    [Google Scholar]
  29. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg Edén C., KÄllenius G., Svenson S. B., Hultberg H., Korhonen T. 1981; Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet ii:1366–1369
    [Google Scholar]
  30. Weiser R., Moffat D. B., Wimpenny J. C., Asscher A. W., Sussman M. 1972; Ethylenediamine tetra-acetic acid (EDTA) induced morphological changes in Proteus mirabilis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A: Medizinische Mikrobiologie und Parasitologie) 222:52–56
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-3-621
Loading
/content/journal/micro/10.1099/00221287-129-3-621
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error