1887

Abstract

During axenic growth, microplasmodia of secreted chitinase and --acetylhexosaminidase. Chitin added to the medium was depolymerized to -acetylglucosamine, but this was not used for growth. Spent culture medium contained three enzymes with activity towards 4-methylumbelliferyl---acetylglucosaminide and these were separated by chromatography on DEAE-cellulose. In previous work --acetylhexosaminidases X and Y were identified. The latter form has now been resolved to yield a new form Z. The Y form can be readily distinguished from both X and Z by its lack of activity towards -diacetylchitobiose. Comparison of --acetylhexosaminidases X and Z revealed differences in thermal stability, pH optima and values. 3,4-Dinitrophenyl-tetra--acetylchitotetraoside is hydrolysed by --acetylhexosaminidase Z, but not by the X form of the enzyme.

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/content/journal/micro/10.1099/00221287-129-3-547
1983-03-01
2021-10-16
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References

  1. Braun V., Behrens K. 1996; A ribonuclease from Physarum. Biochemical properties and synthesis in the mitotic cycle. Biochimica et biophysica acta 195:87–98
    [Google Scholar]
  2. Carlile M. J. 1971; Myxomycetes and other slime moulds. Methods in Microbiology 4:237–265
    [Google Scholar]
  3. Farr D., Horisberger M., Jolles P. 1974; An extracellular rennin-like enzyme produced by Physarum polycephalum. Biochimica et biophysica acta 334:410–416
    [Google Scholar]
  4. Kilpatrick D. C., Stirling J. L. 1975; Two forms of /J-A-acetylhexosaminidase from Physarum polycephalum. Biochemical Society Transactions 3:246–247
    [Google Scholar]
  5. Kilpatrick D. C., Stirling J. L. 1977; Glycosidases from the culture medium of Physarum polycephalum. Biochemical Journal 161:149–160
    [Google Scholar]
  6. Kuehn G. D., Williams R. C., Cox M. K., Jackson J. C. 1977; Intracellular and extracellular β-N-acetylhexosaminidases from Physarumpolycephalum. Archives of Biochemistry and Biophysics 183:686–699
    [Google Scholar]
  7. Molano J., Duran A., Cabib E. 1977; A rapid and sensitive assay for chitinase using tritiated chitin. Analytical Biochemistry 83:648–656
    [Google Scholar]
  8. Reissig J. L., Strominger J. L., Leloir L. F. 1965; A modified colorimetric method for the estimation of N-acetylamino sugars. Journal of Biological Chemistry 217:959–966
    [Google Scholar]
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