1887

Abstract

Trifluoroacetylated whole-cell methanolysates of four strains each of and were analysed by gas chromatography, using a glass capillary column. The major chromatographic peaks were identified by mass spectrometry as derivatives of fatty acids and carbohydrates. In addition, two predominant peaks, present in chromatograms representing , were identified as 2-octadecanol and 2-eicosanol. These secondary alcohols were not found in any of the strains of studied. The amount of tuberculostearic acid in the latter species was less than 1 % of that in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-2-401
1983-02-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/2/mic-129-2-401.html?itemId=/content/journal/micro/10.1099/00221287-129-2-401&mimeType=html&fmt=ahah

References

  1. Asselineau J. 1966 In The Bacterial Lipids p. 69 Edited by Lederer E. Paris: Hermann;
    [Google Scholar]
  2. Guerrant G. O., Lambert M. A., Moss C. W. 1981; Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria. Journal of Clinical Microbiology 13:899–907
    [Google Scholar]
  3. Jantzen E., Frøholm L. O., Hytta R., Bovre K. 1972; Gas chromatography of bacterial whole cell methanolysates. I. The usefulness of trimethylsilyl and trifluoroacetyl derivatives for strain and species characterization. Acta pathologica immunologica et microbiologica scandinavica B80:660–671
    [Google Scholar]
  4. Julak J., Turecek F., Mikova Z. 1980; Identification of characteristic branched-chain fatty acid of Mycobacterium kansasii and gordonae by gas chromatography-mass spectrometry. Journal of Chromatography 190:183–187
    [Google Scholar]
  5. Larsson L., Mårdh P.-A. 1976; Gas chromatographic characterization of mycobacteria: analysis of fatty acids and trifluoroacetylated whole-cell methanolysates. Journal of Clinical Microbiology 3:81–85
    [Google Scholar]
  6. Larsson L., Mårdh P.-A. 1977; Application of gas chromatography to diagnosis of microorganisms and infectious diseases. Acta pathologica immunologica et microbiologica scandinavica supplement 259:5–15
    [Google Scholar]
  7. Larsson L., Bergman R., Mårdh P.-A. 1979a; Gas chromatographic characterization of porcine and human strains belonging to the Mycobacterium avium-intracellulare complex. Acta pathologica immunologica et microbiologica scandinavica B87:205–209
    [Google Scholar]
  8. Larsson L., Mårdh P.-A., Odham G. 1979b; Detection of tuberculostearic acid in mycobacteria and nocardiae by gas chromatography and mass spectrometry using selected ion monitoring. Journal of Chromatography, Biomedical Applications 163:221–223
    [Google Scholar]
  9. Larsson L., Mårdh P.-A., Odham G., Wester-Dahl G. 1980; Detection of tuberculostearic acid in biological specimens by means of glass capillary gas chromatography - electron and chemical ionization mass spectrometry, utilizing selected ion monitoring. Journal of Chromatography, Biomedical Applications 182:402–408
    [Google Scholar]
  10. Larsson L., Mårdh P.-A., Odham G., Wester-Dahl G. 1981; Use of selected ion monitoring for detection of tuberculostearic and C32 mycocerosic acid in mycobacteria and in five-day-old cultures of sputum specimens from patients with pulmonary tuberculosis. Acta pathologica immunologica et microbiologica scandinavica B89:245–251
    [Google Scholar]
  11. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. In Microbiological Classification and Identification pp. 189–256 Edited by Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  12. Odham G., Larsson L., Mårdh P.-A. 1979; Demonstration of tuberculostearic acid in sputum from patients with pulmonary tuberculosis by selected ion monitoring. Journal of Clinical Investigation 63:813–819
    [Google Scholar]
  13. Tisdall P. A., Roberts G. O., Anhalt J. P. 1979; Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone. Journal of Clinical Microbiology 10:506–514
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-2-401
Loading
/content/journal/micro/10.1099/00221287-129-2-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error