1887

Abstract

Fatty acid profiles of 202 coryneform and nocardioform bacteria were recorded by gas chromatography. Strains were grouped according to their profiles using mean linkage cluster analysis and similarity measures based on the correlation coefficient, the angular separation between vectors in a multidimensional space and the degree of overlap between superimposed traces. Comparisons using both real and hypothetical data showed the last of these measures to be the most effective. Strains were divided into two major groups, depending on whether they contained predominantly straight chain or - and -branched acids. The first group was divided into two subgroups according to the relative proportions of the characteristic acids present; one subgroup had six clusters containing the rhodococci, nocardiae, mycobacteria and caseobacters, and the other had two containing the xanthobacters and true corynebacteria. The second group was divided into one subgroup containing strains of and , and one having three clusters. One cluster from this latter subgroup contained cellulomonads, one contained brevibacteria and curtobacteria and one contained arthrobacters, oerskoviae and kurthiae. Identification to generic level by fatty acid composition alone may not be feasible, but fatty acid analysis coupled with morphological examination may be sufficient to identify and the taxon. Distinction is not easy between and the diaminobutyric acid-containing coryneforms and between and

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1983-02-01
2024-12-06
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References

  1. Abe S., Takayama K., Kinoshita S. 1967; Taxonomical studies on glutamic acid-producing bacteria. Journal of General and Applied Microbiology 13:279–301
    [Google Scholar]
  2. Alshamaony L., Goodfellow M., Minnikin D. E., Bowden G. H., Hardie J. M. 1977; Fatty acid and mycolic acid composition of Bacterionema matruchotii and related organisms. Journal of General Microbiology 98:205–213
    [Google Scholar]
  3. Barksdale L., Lanéelle M. A., Pollice M. C., Asselineau J., Welby M., Norgard M. V. 1979; Biological and chemical basis for the reclassification of Microbacterium flavum Orla-Jensen as Corynebacterium flavescens nom. nov. International Journal of Systematic Bacteriology 29:222–233
    [Google Scholar]
  4. Baumgarten J., Reh M., Schlegel H-G. 1974; Taxonomic studies on some Gram-positive coryne-form hydrogen bacteria. Archives of Microbiology 100:207–217
    [Google Scholar]
  5. Block R. J., Durrum G., Zweig G. 1955 A Manual of Paper Chromatography and Paper Electro¬phoresis. New York: Academic Press;
    [Google Scholar]
  6. Bøe B., Gjerde J. 1980; Fatty acid patterns in the classification of some representatives of the families Enterobacteriaceae and Vibrionaceae. Journal of General Microbiology 116:41–49
    [Google Scholar]
  7. Boone C. J., Pine L. 1968; Rapid method for characterisation of actinomycetes by cell wall composition. Applied Microbiology 16:279–284
    [Google Scholar]
  8. Bordet C., Michel G. 1963; Etude des acides gras isoles de plusieurs espèces de Nocardia. Biochimica et biophysica acta 70:613–626
    [Google Scholar]
  9. Bouisset L., Breuillard J., Michel G. 1963; Etude de l’ADN chez les Actinomycetales. Annales de i’Institut Pasteur 104:756–770
    [Google Scholar]
  10. Bousfield I. J. 1972; A taxonomic study of some coryneform bacteria. Journal of General Microbiology 71:441–455
    [Google Scholar]
  11. Bousfield I. J. 1978; The taxonomy of coryneform bacteria from the marine environment. In Coryneform Bacteria pp. 217–233 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  12. Bousfield I. J., Goodfellow M. 1976; The “rhodochrous” complex and its relationships with allied taxa. In The Biology of the Nocardiae pp. 39–65 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  13. Bowie I. S., Grigor M. R., Dunckley D. G., Loutit M. W., Loutit J. S. 1972; The DNA base composition and fatty acid constitution of some Gram-positive pleomorphic soil bacteria. Soil Biology and Biochemistry 4:397–412
    [Google Scholar]
  14. Burton M. O., Lochhead A. G. 1953; Nutritional requirements of Arthrobacter terregens. Canadian Journal of Botany 31:145–151
    [Google Scholar]
  15. Collins M. D. 1978 Lipids in coryneform taxonomy. Ph.D. thesis, University of Newcastle-upon-Tyne, UK
    [Google Scholar]
  16. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. Journal of Applied Bacteriology 48:459–470
    [Google Scholar]
  17. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiological Reviews 45:316–354
    [Google Scholar]
  18. Collins M. D., Goodfellow M., Minnikin D. E. 1979; Isoprenoid quinones in the classification of coryneform and related bacteria. Journal of General Microbiology 110:127–136
    [Google Scholar]
  19. Collins M. D., Goodfellow M., Minnikin D. E. 1980a; Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. Journal of General Microbiology 118:29–37
    [Google Scholar]
  20. Collins M. D., Jones D., Keddie R. M., Sneath P. H. A. 1980b; Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev.; comb. nov. Journal of General Microbiology 120:1–10
    [Google Scholar]
  21. Collins M. D., Jones D., Kroppenstedt R. M. 1981; Reclassification of Corynebacterium ilicis (Mandel, Guba & Litsky) in the genus Arthrobacter, as Arthrobacter ilicis comb. nov. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene(I.Abteilung, Originate C) 2:318–323
    [Google Scholar]
  22. Collins M. D., Goodfellow M., Minnikin D. E. 1982; A survey of the structures of mycolic acids in Corynebacterium and related taxa. Journal of General Microbiology 128:129–149
    [Google Scholar]
  23. Crombach W. H. J. 1978; Caseobacter polymorphus gen. nov., sp. nov., a coryneform bacterium from cheese. International Journal of Systematic Bacteriology 28:354–366
    [Google Scholar]
  24. Cure G. L., Keddie R. M. 1973; Methods for the morphological examination of aerobic coryneform bacteria. In Sampling - Microbiological Monitoring of Environments pp. 123–135 Edited by Board R. G., Lovelock D. W. New York & London: Academic Press;
    [Google Scholar]
  25. De Ley J. 1970; Re-examination of the association between melting point, buoyant density and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology 101:738–754
    [Google Scholar]
  26. Drucker D. B. 1974; Chemotaxonomic fatty acid fingerprints of some streptococci with subsequent statistical analysis. Canadian Journal of Microbiology 20:1723–1728
    [Google Scholar]
  27. Drucker D. B. 1981 Microbiological Applications of Gas Chromatography. Cambridge: Cambridge University Press;
    [Google Scholar]
  28. Drucker D. B., Griffith C. J., Melville T. H. 1976; Fatty acid fingerprints of some chemostat-grown streptococci with computerized data analysis. Microbios Letters 1:31–34
    [Google Scholar]
  29. Federov M. V., Kalininskaya T. A. 1961; The relation of the nitrogen-fixing mycobacterium (Mycobacterium sp. 301) to various carbon sources and to additional growth factors. Mikrobiologiya 30:833–840
    [Google Scholar]
  30. Fulco A. J., Levy R., Bloch K. 1964; The biosynthesis of Δ9-and Δ5-monosaturated (sic) fatty acids by bacteria. Journal of Biological Chemistry 239:998–1003
    [Google Scholar]
  31. Gee J. M., Lund B. M., Metcalf G., Peel J. L. 1980; Properties of a new group of alkalophilic bacteria. Journal of General Microbiology 117:9–17
    [Google Scholar]
  32. Gibson D. M., Ogden I. D. 1979; A rapid method for purifying bacterial deoxyribonucleic acid. Journal of Applied Bacteriology 46:421–423
    [Google Scholar]
  33. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria. Journal of General Microbiology 69:33–80
    [Google Scholar]
  34. Goodfellow M., Alderson G. 1977; The actinomycete-genus Rhodococcus: a home for the “rhodochrous” complex. Journal of General Microbiology 100:99–122
    [Google Scholar]
  35. Goodfellow M., Orlean P. A. B., Collins M. D., Alshamaony L., Minnikin D. E. 1978; Chemical and numerical taxonomy of strains received as Gordona aurantiaca. Journal of General Microbiology 109:57–68
    [Google Scholar]
  36. Goodfellow M., Collins M. D., Minnikin D. E. 1980; Fatty acid and polar lipid composition in the classification of Kurthia. Journal of Applied Bacteriology 48:269–276
    [Google Scholar]
  37. Goodfellow M., Minnikin D. E., Todd C., Alderson G., Minnikin S. M., Collins M. D. 1982; Numerical and chemical classification of Nocardia amarae. Journal of General Microbiology 128:1283–1297
    [Google Scholar]
  38. Guerrant G. O., Lambert M. A., Moss C. W. 1981; Chromatographic analysis of mycolic acid cleavage products in mycobacteria. Journal of Clinical Microbiology 13:899–907
    [Google Scholar]
  39. Gutteridge C. S., Norris J. R. 1979; The application of pyrolysis techniques to the identification of micro-organisms. Journal of Applied Bacteriology 47:5–44
    [Google Scholar]
  40. Hoare D. S., Work E. 1955; The stereoisomers of a, e-diaminopimelic acid: their distribution in nature and behaviour towards certain enzyme preparations. Biochemical Journal 61:562–568
    [Google Scholar]
  41. Ikemoto S., Kuraishi H., Komagata K., Azuma R., Suto T., Murooka H. 1978; Cellular fatty acid composition in Pseudomonas species. Journal of General and Applied Microbiology 24:199–213
    [Google Scholar]
  42. Inoue K., Komagata K. 1976; Taxonomic study on obligately psychrophilic bacteria isolated from Antarctica. Journal of General and Applied Microbiology 22:165–176
    [Google Scholar]
  43. Jantzen E., Bergan T., Bøvre K. 1974a; Gas chromatography of bacterial whole cell methano- lysates.VI. Fatty acid composition of strains within Micrococcaceae. Acta pathologica et microbiologica scandinavica, Section B 82:785–798
    [Google Scholar]
  44. Jantzen E., Bryn K., Bergan T., Bøvre K. 1974b; Gas chromatography of bacterial whole cell methanolysates.V. Fatty acid composition of Neis- seriae and Moraxellae. Acta pathologica et microbiologica scandinavica, Section B 82:769–779
    [Google Scholar]
  45. Jantzen E., Bryn K., Bergan T., Bøvre K. 1975; Gas chromaiography of bacterial whole cell methanolysates.VII. Fatty acid composition of Acinetobacter in relation to the taxonomy of Neisseriaceae. Acta pathologica et microbiologica scandinavica, Section B 83:569–580
    [Google Scholar]
  46. Jones D. 1975; A numerical taxonomic study of coryneform and related bacteria. Journal of General Microbiology 87:52–96
    [Google Scholar]
  47. Jones L. A., Bradley S. G. 1964; Phenetic classification of actinomycetes. Developments in Industrial Microbiology 5:267–272
    [Google Scholar]
  48. Keddie R. M. 1978; What do we mean by coryneform bacteria?. In Coryneform Bacteria pp. 1–12 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  49. Keddie R. M., Bousfield I. J. 1980; Cell wall composition in the classification and identification of coryneform bacteria. In Microbiological Classification and Identification pp. 167–187 Edited by Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  50. Keddie R. M., Cure G. L. 1977; The cell wall composition and distribution of free mycolic acids in named strains of coryneform bacteria and in isolates from various natural sources. Journal of Applied Bacteriology 42:229–252
    [Google Scholar]
  51. Keddie R. M., Cure G. L. 1978; Cell wall composition of coryneform bacteria. In Coryneform Bacteria pp. 47–83 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  52. Kroppenstedt R. M., Kutzner H. J. 1978; Biochemical taxonomy of some problem actinomycetes. Zentrablatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (I. Abteilung) Suppl. 6:125–133
    [Google Scholar]
  53. Lanéelle M-A., Asselineau J., Welby M., Norgard M. V., Imaeda T., Pollice M. C., Barksdale L. 1980; Biological and chemical bases for the reclassification of Brevibacterium vitarumen (Bechdel et al.) Breed (Approved Lists 1980) as Corynebacterium vitarumen (Bechdel et al.) comb, nov. and Brevibacterium liquefaciens Okabayashi & Masuo (Approved Lists 1980) as Corynebacterium liquefaciens (Okabayashi & Masuo) comb. nov. International Journal of Systematic Bacteriology 30:539–546
    [Google Scholar]
  54. Lechevalier M. P. 1977; Lipids in bacterial taxonomy - a taxonomist’s view. CRC Critical Reviews in Microbiology 5:109–210
    [Google Scholar]
  55. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochemical Systematics and Ecology 5:249–260
    [Google Scholar]
  56. Malik K. A., Claus D. 1979; Xanthobacter flavus, a new species of nitrogen-fixing hydrogen bacteria. International Journal of Systematic Bacteriology 29:283–287
    [Google Scholar]
  57. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  58. Metcalfe L. D., Schmitz A. A. 1961; The rapid preparation of fatty acid esters for gas chromatographic analysis. Analytical Chemistry 33:363–364
    [Google Scholar]
  59. Minnikin D. E., Goodfellow M. 1976; Lipid composition in the classification and identification of nocardiae and related taxa. In The Biology of the Nocardiae pp. 160–169 Edited by Goodfellow M., Brownell M., Serrano J. A. London: Academic Press;
    [Google Scholar]
  60. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. In Microbiological Classification and Identification pp. 189–256 Edited by Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  61. Minnikin D. E., Goodfellow M., Collins M. D. 1978; Lipid composition in the classification and identification of coryneform bacteria and related taxa. In Coryneform Bacteria pp. 85–160 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  62. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. Journal of Applied Bacteriology 47:87–95
    [Google Scholar]
  63. Nesterenko O. A., Andreev L. V., Nogina T. M., Shkaruba V. U. 1980; Fatty acid composition of some coryneform and nocardioform bacteria. Mikrobiologicheskii zhurnal 42:288–293
    [Google Scholar]
  64. Orchard V. A., Goodfellow M., Williams S. T. 1977; Selective isolation and occurrence of nocardiae in soil. Soil Biology and Biochemistry 9:233–238
    [Google Scholar]
  65. Pommier M. T., Michel G. 1973; Phospholipid and acid composition of Nocardia and nocardoid bacteria as criteria of classification. Biochemical Systematics 1:3–12
    [Google Scholar]
  66. Randerath K. 1963 Thin Layer Chromatography (English translation) New York & London: Academic Press;
    [Google Scholar]
  67. Schleifer K-H., Kändler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  68. Sharpe M. E., Law B. A., Phillips B. A. 1976; Coryneform bacteria producing methanethiol. Journal of General Microbiology 94:430–435
    [Google Scholar]
  69. Sharpe M. E., Law B. A., Phillips B. A., Pitcher D. G. 1977; Methanethiol production by coryneform bacteria: strains from dairy and human skin sources and Brevibacterium linens. Journal of General Microbiology 101:345–349
    [Google Scholar]
  70. Skerman V. B. D., Mcgowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  71. Sokal R. R., Michener C. D. 1958; A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 38:1409–1438
    [Google Scholar]
  72. Souza K. A., Deal P. H. 1977; Characterisation of a novel extremely alkalophilic bacterium. Journal of General Microbiology 101:103–109
    [Google Scholar]
  73. Stackebrandt E., Kändler O. 1979; Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology, and proposal of seven neotype strains. International Journal of Systematic Bacteriology 29:273–282
    [Google Scholar]
  74. Stackebrandt E., Häringer M., Schleifer K-H. 1980; Molecular genetic evidence for the transfer of Oerskovia species into the genus Cellulomonas. Archives of Microbiology 127:179–185
    [Google Scholar]
  75. Suzuki K., Kaneko T., Komagata K. 1981; Deoxyribonucleic acid homologies among coryneform bacteria. International Journal of Systematic Bacteriology 31:131–138
    [Google Scholar]
  76. Tsukamura M., Mizuno S., Tsukamura S., Tsukamura J. 1979; Comprehensive numerical classification of 369 strains of Mycobacterium, Rhodococcus and Nocardia. International Journal of Systematic Bacteriology 29:110–129
    [Google Scholar]
  77. Weigel J., Wilke D., Baumgarten J., Opitz R., Schlegel H-G. 1978; Transfer of the nitrogen¬fixing hydrogen bacterium Corynebacterium auto- trophicum Baumgarten et al. to Xanthobacter gen. nov. International Journal of Systematic Bacteriology 28:573–581
    [Google Scholar]
  78. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria III. DNA base composition of coryneform bacteria. Journal of General and Applied Microbiology 16:215–224
    [Google Scholar]
  79. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria V. Classification of coryneform bacteria. Journal of General and Applied Microbiology 18:417–431
    [Google Scholar]
  80. Yamada K., Inouye G., Tahara Y., Kondo K. 1976; The menaquinone system in the classification of coryneform and nocardioform bacteria and related organisms. Journal of General and Applied Microbiology 22:203–214
    [Google Scholar]
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