1887

Abstract

A lysogen of K12 with integrated into was induced and the lysates plated on a Pel host [on which strains with less than the wild-type amount of DNA form plaques at low frequency ( Cameron ., 1977 )]. All of the 40 plaques examined contained phage able to transduce at least two of the genes known from bacteriophage P1 transduction experiments to be closely linked to Assuming that each specialized transducing phage arose by a single illegitimate recombination event, the distribution of phage types showed that the gene order is () ; both and were dominant. Analysis of restriction endonuclease digests of the transducing phage confirmed that no unexpected DNA rearrangements had taken place and allowed the construction of a map of the sites of action of the restriction endonucleases , dIII, and for over 20 kilobases of DNA.

In an Appendix, we show and mutants to be deficient in sulphate assimilation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-2-349
1983-02-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/2/mic-129-2-349.html?itemId=/content/journal/micro/10.1099/00221287-129-2-349&mimeType=html&fmt=ahah

References

  1. Ashworth J. M., Kornberg H. L. 1966; Iheanaplerotic fixation of carbon dioxide by Escherichia coli. Proceedings of the Royal Society B165:179–188
    [Google Scholar]
  2. Bachmann B. J., Low K. B. 1980; Linkage map of Escherichia coli K12, edition 6. Microbiological Reviews 44:1–56
    [Google Scholar]
  3. Boronat A., Jones-Mortimer M. C., Kornberg H. L. 1982; A specialized transducing phage, ApsrlA, for the sorbitol phosphotransferase of Escherichia coli K12. Journal of General Microbiology 128:605–611
    [Google Scholar]
  4. Cameron J. R., Philippson P., Davies R. W. 1977; Analysis of chromosomal integration and deletions of yeast plasmids. Nucleic Acids Research 4:1429–1448
    [Google Scholar]
  5. Cordaro C. 1976; Genetics of the bacterial phosphoenolpyruvate: glycosephosphotransferase system. Annual Review of Genetics 10:341–359
    [Google Scholar]
  6. Davies R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics. Cold Spring Harbor:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  7. Enquist L. W., Weisberg R. A. 1976; The red plaque test: a rapid method for identification of excision-defective variants of bacteriophage lambda. Virology 72:147–153
    [Google Scholar]
  8. Gottesman M. E., Yarmolinsky M. B. 1968; Integration-negative mutants of bacteriophage lambda. Journal of Molecular Biology 31:487–505
    [Google Scholar]
  9. Gottesman M. M. 1976; Isolation and characterisation of a λspecialised transducing phage for the Escherichia coli DNA ligase gene. Virology 72:33–44
    [Google Scholar]
  10. Kornberg H. L., Watts P. D. 1979; tgs and err genes involved in catabolite inhibition and inducer exclusion in Escherichia coli. FEBS Letters 104:313–328
    [Google Scholar]
  11. Lindahl G., Sironi G., Bialy H., Calendar R. 1970; Bacteriophage lambda: abortive infection of bacteria lysogenic tor phage P2. Proceedings ojtrie National Academy of Sciences of the United States of America 66:587–594
    [Google Scholar]
  12. Miller J. H. 1972 Experiments in molecular genetics. Cold Spring Harbor:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  13. Parra F., Jones-Mortimer M. C., Kornberg H. L. 1983; Phosphotransferase-mediated regulation of carbohydrate utilization in Escherichia coli K12: the nature of the iex (err) and gsr (tgs) mutations. Journal of General Microbiology 129:337–348
    [Google Scholar]
  14. Reif H. J., Saedler H. 1977; Chromosomal rearrangements in the gal region of E. coli K12. In DNA Insertion Elements, Plasmids and Episomes pp. 81–91 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  15. Schrenk W. J., Weisberg R. A. 1975; A simple method for making new transducing lines of coli- phage λ. Molecular and General Genetics 137:101–107
    [Google Scholar]
  16. Shimada K., Weisberg R. A., Gottesman M. E. 1972; Prophage lambda at unusual chromosomal locations. I. Location of secondary attachment sites and properties of lysogens. Journal of Molecular Biology 63:483–503
    [Google Scholar]
  17. Swanstrom M., Adams M. H. 1951; Agar layer method for production of high titer phage stocks. Proceedings of the Society for Experimental Biology and Medicine 78:372–375
    [Google Scholar]
  18. Szybalski E. H., Szybalski W. 1979; A comprehensive molecular map of bacteriophage lambda. Gene 7:217–270
    [Google Scholar]
  19. White R. J., Kent P. W. 1970; An examination of the inhibitory effects of N-iodoacetylglucosamine on Escherichia coli and isolation of resistant mutants. Biochemical Journal 118:81–87
    [Google Scholar]
/content/journal/micro/10.1099/00221287-129-2-349
Loading
/content/journal/micro/10.1099/00221287-129-2-349
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error