%0 Journal Article %A Mergeay, Max %A Gerits, Jozef %T Transduction of Escherichia coli trp Genes in Salmonella typhimurium and Effect of N-Methyl-N′-Nitro-N-Nitrosoguanidine on Transduction with Heterogenotic DNA %D 1983 %J Microbiology, %V 129 %N 2 %P 321-335 %@ 1465-2080 %R https://doi.org/10.1099/00221287-129-2-321 %I Microbiology Society, %X SUMMARY: P1Kc-mediated transduction of Escherichia coli trp genes occurred at a frequency of about 10−8 in Salmonella typhimurium trp strains carrying mutations determining sensitivity to P1 and a low level of restriction enzymes. Heterospecific transductants were analysed by using them as donors in second-stage transductions mediated by bacteriophage KB int. One class of heterospecific transductants had the phenotype Trp+ Pro− but were extremely unstable and reverted at high frequency (up to 80%) to the parental phenotype. The Trp+ Pro− phenotype probably represents insertions of the E. coli trp genes in the S. typhimurium pro genes. It was stable in a RecA background. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-2-321